Determination of polycyclic aromatic hydrocarbons, azaarenes, and aminoazaarenes in meat samples by solid-phase extraction (SPE) and HPLC

• Autorzy: Warzecha L. , Stróżyk M. , Janoszka B. , Błaszczyk U. , Bodzek D.
• Języki publikacji: EN

Abstrakty

EN

A study has been conducted to determine the optimum conditions for selective enrichment, by use of coupled solid-phase extraction (SPE) cartridges, of mutagenic and/or carcinogenic organic compounds from meat samples, before qualitative and/or quantitative analysis. Such compounds, generated during heat-processing of meat, can be polycyclic aromatic hydrocarbons (PAH), polycyclic aromatic heterocyclic nitrogen compounds (azaarenes, PANH), or heterocyclic aromatic amines (HAA). To determine the optimum conditions for the clean-up procedure three kinds of Extrelut-type diatomaceous earth columns were tested. The aminoazaarene fraction was analyzed by reversed-phase high-performance liquid chromatography (RP HPLC) on a chemically bonded C8 column and isocratic elution with 10% (v/v) acetonitrile in triethylamine-phosphate buffer (pH 3.2). Analysis of the PAH and PANH fractions was performed by RP HPLC on a C18 column with acetonitrile-water, 84:16 (v/v), as mobile phase. The identities of HPLC peaks were confirmed by GC-MS for both PAH and PANH. The clean-up procedure was used to analyze samples of roasted chicken breast meat. The aminoazaarenes 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3,4,8-tri-methylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx), and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) were detected in such samples, at total levels from 8.6-13.9 ng g-1. Polycyclic aromatic hydrocarbons identified included benzo(a)anthracene (6.5-7.5 ng g-1) and benzo(a)pyrene (4.9-7.6 ng g-1). Among the azaarenes benzo(a)acridine was found at concentrations of 10.3-12.9 ng g-1.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2002
• Tom: No. 12
• Strony: 104--121
• Opis fizyczny: Bibliogr. 17 poz., rys., tab.

Twórcy

autor

Warzecha L.

• Medical University of Silesia, Faculty of Medicine, Department of Chemistry, Jordana 19, 41-808 Zabrze, Poland

autor

Stróżyk M.

• Department of Molecular Biology, Centre of Oncology, M. Skłodowska – Curie Institute, Wybrzeże Armii Krajowej 15, 44-101 Gliwice, Poland

autor

Janoszka B.

• Medical University of Silesia, Faculty of Medicine, Department of Chemistry, Jordana 19, 41-808 Zabrze, Poland

autor

Błaszczyk U.

• Medical University of Silesia, Faculty of Medicine, Department of Biochemistry, Jordana 19, 41-808 Zabrze, Poland

autor

Bodzek D.

• Medical University of Silesia, Faculty of Medicine, Department of Chemistry, Jordana 19, 41-808 Zabrze, Poland

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