TLC analysis of glucose and maltose in Biomphalaria glabrata (Gastropoda) infected with Schistosoma mansoni (Trematoda)

• Autorzy: Schariter J. J. , Fried B. , Sherma J.
• Języki publikacji: EN

Abstrakty

EN

Analysis by thin-layer chromatography (TLC) has been performed on the hemolymph and digestive gland-gonad complex (DDG) of Biompha-laria glabrata snails experimentally infected with Schistosoma mansoni larvae. The major sugars identified in both the hemolymph and DGG of infected and uninfected snails were glucose and maltose. Densitometric quantification showed that there was a significant decrease (Student's t-test, P<0.05) in levels of glucose and maltose in the hemolymph of infected snails 5 and 7 weeks post-infection. At week 7 after infection there was a significant decrease in maltose in the DGG of infected snails. Findings from this work were compared with results previously obtained from spectrophotometric analysis of glucose in B. glabrata infected with larval S. mansoni. It was found that spectrophotometric analysis was less selective and reliable than TLC.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2001
• Tom: No. 11
• Strony: 102--107
• Opis fizyczny: Bibliogr. 8 poz., rys., tab.

Twórcy

autor

Schariter J. J.

autor

Fried B.

autor

Sherma J.

• Department of Chemistry, Lafayette College, Easton, PA 18042, USA