Separation and estimation of bisphenol a by liquid chromatography with UV detection

• Autorzy: Nagahashi M. , Kikuchi N. , Migaki E. , Watanabe S. , Miki T.
• Języki publikacji: EN

Abstrakty

EN

Reliable HPLC separation and estimation of bisphenol A has been performed on a conventional ODS column with 40:60 acetonitrile–water as mobile phase and UV detection at 277 nm. Linear regression plots were obtained for the dependence of response on bisphenol A concentration in the working ranges 2 to 10 ng and 10 to 100 ng; the correlation coefficients for the plots were r = 0.9976 (n = 5) and r = 0.9987 (n = 6), respectively. Aqueous samples were applied to a solid-phase extraction cartridge packed with three layers, Bio-Rad AG 50W X8 [H+], ODS–silica, and Bio-Rad AG 1 X8 [formate–]. Eluent from this cartridge was passed through a normal-phase Florisil PR cartridge. These isolation and purification cartridges effectively separated and purified bisphenol A from spiked samples with recovery between 86 and 92%. The method was used to estimate bisphenol A in water samples.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2000
• Tom: No. 10
• Strony: 110--122
• Opis fizyczny: Bibliogr. 10 poz., rys., tab.

Twórcy

autor

Nagahashi M.

autor

Kikuchi N.

autor

Migaki E.

autor

Watanabe S.

autor

Miki T.

• Laboratory of Public Health Nutrition and Epidemiology, Department of Nutritional Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture