HPLC study of the modification of haemoglobin by formaldehyde

• Autorzy: Ostrowska J. , Roszkowska A. , Skrzydlewska E. , Figaszewski Z. A.
• Języki publikacji: EN

Abstrakty

EN

Formaldehyde is known to react with proteins. The effect of formaldehyde on the total charge and on the tryptic map of haemoglobin has been analysed by HPLC. The effect of methanol intoxication on the total charge of rat haemoglobin was also determined. The total charge of haemoglobin was examined on a Mono Q column by gradient elution with solutions of Tris–HCl and Tris–HCl–NaCl. Tryptic maps were determined on a C18 column by gradient elution with solutions of TFA–water and TFA–acetonitrile–water. Formaldehyde in vitro and methanol intoxication resulted in prolonged retention of haemoglobin. Formaldehyde also reduces the susceptibility of haemoglobin to trypsin hydrolase which leads to a reductions in the magnitudes of peaks in the tryptic map.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 1999
• Tom: No. 9
• Strony: 87--96
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Ostrowska J.

autor

Roszkowska A.

autor

Skrzydlewska E.

autor

Figaszewski Z. A.

• Laboratory of Electrochemistry, Institute of Chemistry, The University of Białystok, Al. J. Piłsudskiego 11/4, 15-443 Białystok, Poland