TLC and GC-MS identification of glucose and maltose in Biomphalaria glabrata (Gastropoda), and use of quantitative TLC to determine the effect of starvation on the amounts of these carbohydrates

• Autorzy: Cline D. J. , Fried B. , Sherma J.
• Języki publikacji: EN

Abstrakty

EN

Maltose and glucose have been identified as the primary carbohydrates in the planorbid snail Biomphalaria glabrata by high performance thin layer chromatography (HPTLC) on silica gel, amino-bonded, C18 reversed-phase, and cellulose plates. The use of specific detection reagents for reducing sugars, spiking experiments, and differential coloration of sugar zones with ?-naphthol reagent on amino plates aided the identification of glucose and maltose. Gas chromatography–mass spectrometry (GC–MS) analysis confirmed the identification maltose and glucose on the basis of retention times and spectral fingerprints. The presence of trehalose was not confirmed. After identifying maltose and glucose, snails were starved for twelve days and the effects on the concentrations of these sugars were determined. Amounts of glucose dropped significantly in the digestive gland–gonad complex (DGG) from day 0 to day 12; maltose in the hemolymph and DGG and glucose in the hemolymph did not change significantly during the starvation period.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 1999
• Tom: No. 9
• Strony: 79--86
• Opis fizyczny: Bibliogr. 11 poz., tab.

Twórcy

autor

Cline D. J.

autor

Fried B.

autor

Sherma J.

• Department of Chemistry, Lafayette College, Easton, PA 18042, USA