Paper electrophoretic separation of phenolic compounds with acetate and citrate buffer electrolytes: determination of some important phenolic compounds in blood

• Autorzy: Nabi S. A. , Sikarwar A. , Gupta A.
• Języki publikacji: EN

Abstrakty

EN

Paper electrophoresis of 35 phenolic compounds has been performed with acetate and citrate buffers of varying pH. The study revealed a number of important binary and ternary separations. A few important quantitative separations were achieved: (i) resorcinol from quinol, catechol, coumaric acid, 3,4-dihydroxycinnamic acid and pyrogal-lol; (ii) a-naphthol from b-naphthol; and (iii) 4-nitrophenol from 2-nitro-phenol, 4-aminophenol, and picric acid. A new method is reported for rapid sample preparation for determination of free phenolic compounds and a few conjugated phenolic compounds in blood. Simultaneous separa-tion and determination of five phenolic compounds, quinol, resorcinol, coumaric acid, catechin and 3,4-dihydroxycinnamic acid was achieved by use of pH 3.67 citrate buffer; separation of gallic acid and 4-aminophenol was achieved by use of pH 3.62 acetate buffer. These results were then correlated with the general health and habits of the persons from whom the blood was taken. The accuracy and reproducibility of the method has been checked statistically. The limit of detection was found to be 0.20 μg L-1.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 1998
• Tom: No. 8
• Strony: 162--174
• Opis fizyczny: Bibliogr. 12 poz., rys., tab.

Twórcy

autor

Nabi S. A.

autor

Sikarwar A.

autor

Gupta A.

• Analytical Division, Department of Chemistry, Aligarh Muslim University, Aligarh-202002, India