Tlc and GC - MS determination of cholesterol in consumable fats

• Autorzy: Bodzek D. , Bąkowski W. , Wielkoszyński T. , Janoszka B. , Jaremczuk B. , Tarnawski R. , Tyrpień K.
• Języki publikacji: EN

Abstrakty

EN

Cholesterol is one of the more important compounds causing arteriosclerosis and other illnesses of the circulatory system. In this work we have developed a procedure for separating a mixture of sterols from commercial consumable fats and, as the next step, identifying the main components of the sterol mixture. The method of separation included conventional and over-pressure thin-layer chromatography and solid-phase extraction (SPE); capillary gas chromatography with mass spectrometric detection was the main method of identification. Sterols and sterol esters were separated from the fats by solid-phase extraction on Bakerbond aminopropyl or silica gel columns. Preli-minary identification of the isolated sterols was achieved by TLC in a DS-sandwich type chamber. Full identification of the isolated sterols was performed by GC-MS and quantitative estimation of the cholesterol content by capillary GC with flame ionization detection. The amount of sterols was found to be in the range 4.01-11.46 mg g-1 fat; the amount of cholesterol was 0-5.14 mg g-1 fat.

Słowa kluczowe

EN

sterol mixture; thin-layer chromatography; solid-phase extraction; cholesterol

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 1998
• Tom: No. 8
• Strony: 122--143
• Opis fizyczny: Bibliogr. 13 poz., rys.

Twórcy

autor

Bodzek D.

autor

Bąkowski W.

autor

Wielkoszyński T.

autor

Janoszka B.

autor

Jaremczuk B.

autor

Tarnawski R.

autor

Tyrpień K.

• Silesian Medical Academy, Department of General Chemistry, Jordana str 19. 41-808 Zabrze, Poland