Modelling of solute retention in normal-phase HPLC with the chemically bonded 3-cyanopropyl stationary phase

• Autorzy: Prus W. , Vander Heyden Y. , Massart D. L. , Kowalska T.
• Języki publikacji: EN

Abstrakty

EN

We have investigated the applicability of two models of solute retention (i.e. Models I and II, devised by Kowalska for liquid chromato-graphy with alcohol-hydrocarbon mobile phases) for description of the chromatographic behavior of 25 test solutes in normal-phase (NP) HPLC systems with the 3-cyanopropyl stationary phase and 2-propanol-n-hexane as mobile phase. The test solutes employed were from five chemical classes, (i) an alkylbenzene, (ii) crown ethers, (iii) hydroxyaromatic and methylhydroxyaromatic compounds, (iv) naphthylamines and (v) quino-line and its derivatives, and thus differed considerably in molecular structure, and hence in their ability to compete with 2-propanol molecules for active sites (i.e. the -CN and ≡Si-OH groups) of the stationary phase, which is the quintessence of the so-called displacement mechanism of solute retention. The performance of Models I and II was evaluated in terms of the numerical values of the root-mean-squared error (RMSt), calculated for each employed test solute and our investigations showed that Models I and II are very well suited for description of the chromato-graphic behavior of solutes for which there is a non-linear empirical relationship between retention parameter (RF or lnk) and the composition of a binary mobile phase.

Słowa kluczowe

EN

high-performance liquid chromatographic

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 1998
• Tom: No. 8
• Strony: 98--108
• Opis fizyczny: Bibliogr. 5 poz., rys., tab.

Twórcy

autor

Prus W.

autor

Vander Heyden Y.

autor

Massart D. L.

autor

Kowalska T.

• Faculty of Textile Engineering and Environmental Protection, Technical University of Łódź Branch in Bielsko-Biała, 2 Willowa Street, 43-309 Bielsko-Biała, Poland