Peptide mapping by high-performance liquid chromatography

• Autorzy: Skrzydlewska E. , Ostrowska J. , Figaszewski Z.
• Języki publikacji: EN

Abstrakty

EN

The determination of protein structure by peptide mapping is described in this paper. The method consists in selective fragmentation of the protein molecule with trypsin, separation of resulting peptides by HPLC, and amino acid analysis. Gradient reversed-phase HPLC is performed with 0.1% TFA in water and 0.1% TFA in acetonitrile as mobile phases.

Słowa kluczowe

EN

peptide mapping; high-performance liquid chromatographic

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 1998
• Tom: No. 8
• Strony: 70--84
• Opis fizyczny: Bibliogr. 68 poz., rys., tab.

Twórcy

autor

Skrzydlewska E.

autor

Ostrowska J.

autor

Figaszewski Z.

• Department of Instrumental Analysis, Medical Academy, 15-230 Białystok 8, Poland