Effects of pchloromercuribrnzoate and n-ethylmaleimide on hemolysis under hydrostatic pressure of human erythocytes

• Autorzy: Yamaguchi T. , Terada S.
• Języki publikacji: EN

Abstrakty

EN

The relation between high-pressure (200mpa)-induced hemolisis and spectrin tetramer-dimer equilibrium was examined by using human erythrocytes treatedwith N-ethylmaleimide (NEM) and p-chloromercuribenzoate (PCMB). The values of hemolysis at 200 Mpa of NEM-treated erythrocytes increased upon mild modification of membrane SH groups, but decreased upon severe modification. In both cases, the membrane structure of NEM-treated cells became more stable against high pressure upon further incubation at 370C in reagent-free buffer. In PCMB-treated erythrocytes, the cells were hemolyzed completely at 200 Mpa. On the other hand, the concentration of spectrin dimers increased upon treatment with NEM, but remained constant for further incubation at 370C. In the case of PCMB-treated cells, spectrin tetramers did not dissociate into dimers as with NEM. These results suggest that hemolytic properties at 200 Mpa in erytrocytes treated with SH-reactive reagents are inexplicable in terms of the dissociation only of spectrin.

• Wydawca: Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej
• Czasopismo: Polish Hyperbaric Research
• Rocznik: 2005
• Tom: Nr 1(10)
• Strony: 44--43
• Opis fizyczny: Bibliogr. 9 poz.

Twórcy

autor

Yamaguchi T.

• Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-0180, Japan

autor

Terada S.

• Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-0180, Japan

Bibliografia

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