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Języki publikacji
Abstrakty
The relation between high-pressure (200mpa)-induced hemolisis and spectrin tetramer-dimer equilibrium was examined by using human erythrocytes treatedwith N-ethylmaleimide (NEM) and p-chloromercuribenzoate (PCMB). The values of hemolysis at 200 Mpa of NEM-treated erythrocytes increased upon mild modification of membrane SH groups, but decreased upon severe modification. In both cases, the membrane structure of NEM-treated cells became more stable against high pressure upon further incubation at 370C in reagent-free buffer. In PCMB-treated erythrocytes, the cells were hemolyzed completely at 200 Mpa. On the other hand, the concentration of spectrin dimers increased upon treatment with NEM, but remained constant for further incubation at 370C. In the case of PCMB-treated cells, spectrin tetramers did not dissociate into dimers as with NEM. These results suggest that hemolytic properties at 200 Mpa in erytrocytes treated with SH-reactive reagents are inexplicable in terms of the dissociation only of spectrin.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
44--43
Opis fizyczny
Bibliogr. 9 poz.
Twórcy
autor
- Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-0180, Japan
autor
- Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-0180, Japan
Bibliografia
- 1. Kitajima H., Yamaguchi T. and Kimoto E. (1990): Hemolysis of human erythrocytes under hydrostatic pressure is suppressed by cross-linking of membrane proteins; J. Biochem., 108, 1057-1062.
- 2. Yamaguchi T., Kajikawa T. and Kimoto E. (1991): Vesiculation induced by hydrostatic pressure in human erythrocytes; J. Biochem., 110,355-359.
- 3. Yamaguchi T., Kawamura H., Kimoto E. and Tanaka M. (1989):Effects of temperature and pH on hemoglobin release from hydrostatic pressure-treated erythrocytes; J. Biochem., 106, 1080-1085.
- 4. Ungewickell E. and Gratzer W. (1978): Self-association of human spectrin: A thermodynamic and kinetic study; Eur. J. Biochem., 88, 379-385.
- 5. Yamaguchi T. and Terada S. (1999): Effects of thiol-reactive reagents on hemolysis under hydrostatic pressure of human erythrocytes; in High Pressure Bioscience and Biotechnology Elsevier Science Publishers B.V., Amsterdam.
- 6. Dodge J.T., Mitchell C. and Hanahan D.J. (1963): The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes; Arch. Biochem. Biophys., 100, 119-130.
- 7. Yamaguchi T., Matsumoto M. and Kimoto E. (1993): Hemolytic properties under hydrostatic pressure of neuraminidase- or protease-treated humanerythrocytes; J. Biochem., 114, 576-581
- 8. Yamaguchi T., Murata Y., Kobayashi J. and Kimoto E. (1994): Effects ofchemical modification of membrane thiol groups on hemolysisof human erythrocytes under hydrostatic pressure; Biochim. Biophys.Acta,1195, 205-210.
- 9. Knepper M. A. (1994): The aquaporin family of molecular water channels; Proc. Natl. Acad. Sci. USA, 91, 6255-6258.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BAT1-0028-0042