Synthesis of human heat shock protein 70 (29-42) fragment and evaluation of its immunogenicity

• Autorzy: Karawajczyk B. , Wysocki J. , Kunikowska D. , Maćkiewicz Z. , Głośnicka R. , Korzeniowski A. , Górski J. , Kupryszewski G.
• Języki publikacji: EN

Abstrakty

EN

The 29-42 fragment of human heat shock protein 70 (hsp 70) was synthesized by the solid phase method. As determined by enzyme-linked immunosorbent assay (ELISA), the synthetic hsp 70(29-42) peptide generated relatively strong immune response in immunized rabbits. Antibody titers were comparable with anti hsp 70 antibody serum level that was induced by immunization with recombinant protein (hsp 70). It was established that antibodies directed against hsp 70(29-42) peptide could be applied in ELISA for detecting hsp 70 in body fluids and tissues.

Słowa kluczowe

EN

solid phase peptide synthesis; human heat shock protein 70 fragments; immunogenicity

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 1998
• Tom: Vol. 72, nr 6
• Strony: 1017--1020
• Opis fizyczny: Bibliogr. 11 poz., tab.

Twórcy

autor

Karawajczyk B.

autor

Wysocki J.

autor

Kunikowska D.

autor

Maćkiewicz Z.

autor

Głośnicka R.

autor

Korzeniowski A.

autor

Górski J.

autor

Kupryszewski G.

• Faculty of Chemistry University of Gdańsk 80-952 Gdańsk Poland