Proinsulin C-peptides from bovine, ovine and bisontine pancreata

• Autorzy: Izdebski J. , Fiertek D. , Majewski T.
• Języki publikacji: EN

Abstrakty

EN

Two C-peptides corresponding to sequences of genetic variants of bovine proinsulin have been synthesized by solid-phase method. C-peptide containing material has been isolated from two bovine pancreata using acid-ethanol extraction, gel filtration on Sephadex G-50 and ion exchange chromatography on carboxymethyl cellulose. Comparison of these materials with synthetic standards on reverse-phase HPLC have revealed that both pancreata contained only one variant of C-peptide which had Pro in position 16. The second variant of bovine proinsulin C-peptide with Leu at this position has not been detected. The corresponding genetic variant of bovine proinsulin, containing Leu within C-peptide sequence, has been found by other authors in the material obtained from a pool of large number of pancreata. Our results exclude the possibility that the two bovine proinsulins are a result of expression of two insulin genes in the bovine species as a whole. The same C-peptide was found in ovine and bisontine pancreata.

Słowa kluczowe

EN

Bison bonasus; bisontine C-peptide; Bovidae family; genetic variant; ovine C-peptide; peptide synthesis; proinsulin

• Wydawca: Polskie Towarzystwo Chemiczne
• Czasopismo: Polish Journal of Chemistry
• Rocznik: 1998
• Tom: Vol. 72, nr 3
• Strony: 548--553
• Opis fizyczny: Bibliogr.10 poz., wykr.

Twórcy

autor

Izdebski J.

autor

Fiertek D.

autor

Majewski T.

• Laboratory of Peptides Department of Chemistry University of Warsaw 02-093 Warsaw Pasteura 1 Poland,