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Two C-peptides corresponding to sequences of genetic variants of bovine proinsulin have been synthesized by solid-phase method. C-peptide containing material has been isolated from two bovine pancreata using acid-ethanol extraction, gel filtration on Sephadex G-50 and ion exchange chromatography on carboxymethyl cellulose. Comparison of these materials with synthetic standards on reverse-phase HPLC have revealed that both pancreata contained only one variant of C-peptide which had Pro in position 16. The second variant of bovine proinsulin C-peptide with Leu at this position has not been detected. The corresponding genetic variant of bovine proinsulin, containing Leu within C-peptide sequence, has been found by other authors in the material obtained from a pool of large number of pancreata. Our results exclude the possibility that the two bovine proinsulins are a result of expression of two insulin genes in the bovine species as a whole. The same C-peptide was found in ovine and bisontine pancreata.
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Tom
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548--553
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Bibliogr.10 poz., wykr.
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- Laboratory of Peptides Department of Chemistry University of Warsaw 02-093 Warsaw Pasteura 1 Poland, izdebski@chem.uw.edu.pl
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bwmeta1.element.baztech-article-BAR2-0001-0074