Selection and optimization of staining techniques of Chinese hamster lung fibroblast V79 cell preparations

• Autorzy: Rudnicka K. , Krzyżewski Z.
• Języki publikacji: EN

Abstrakty

EN

We present the results of optimization of fixation and staining of the Chinese hamster lung fibroblast V79 cell line. The experiment included four methods of fixation and three methods of staining and enabled the selection of the best visualization technique for V79 cell lines culture. The Schiff method of colouring with Light Green contrasted well the cell nucleus and the cytoplasm, but the staining was light-sensitive and transient. The Giemsa staining made cell preparations suitable for microscopic evaluation, although the difference between the nucleus and the cytoplasm was not always easily recognisable.

Słowa kluczowe

EN

cell line V79; Giemsa; Schiff; Light Green

PL

linia komórkowa V79; barwnik Giemsy; Schiff; Light Green

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2011
• Tom: Vol. 7, No. 2
• Strony: 84--87
• Opis fizyczny: Bibliogr. 2 poz., fot.

Twórcy

autor

Rudnicka K.

autor

Krzyżewski Z.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn-Kortowo, Sloneczna 45G, Poland, Phone: [phone],

Bibliografia

• http://brain.fuw.edu.pl/edu/BIOL:Wst%C4%99p_Mikroskopia_oraz_technika_histologiczna, 25.11.2011.
• ISO 21427-2, Water Quality - Evaluation of Genotoxicity by Measurement of Induction of Micronuclei. - Part 2: Mixed Population Method Using the Cell Line V79.