Quantification of triazine herbicides using chloroplasts in conjunction with thin-layer chromatography

• Autorzy: Broszat M. , Ernst H. , Spangenberg B.
• Języki publikacji: EN

Abstrakty

EN

We present a video-densitometric quantification method for the triazine herbicides atraton, terbumeton, simazine, atrazine and terbutylazine. Triazine herbicides were separated on silica gel using methyl-t-butyl ether, cyclohexane (1+1, v/v) as mobile phase. The quantification was based on a bioeffective-linked analysis using chloroplast and 2,6-dichlorophenolindophenol. Within 1-2 minutes HILL-reaction inhibitor substances show blue-grey zones on a pale yellowgreen background. To increase the contrast, the moist plate can be dipped into a solution of PEG-600 (10% PEG-600 in methanol) for 2s. Measurements were carried out using a 16 bit ST-1603ME CCD camera with 1.56 megapixels (from Santa Barbara Instrument Group, Inc., Santa Barbara, USA). A white LED was used for illumination purposes. The range of linearity covers more than one magnitude using the (1/R) - 1 expression data transformation. The method can be used for herbicide screenings in environmental samples, because not spectral sensitivity but herbicide activity is measured. The separation method is cheap, fast and reliable.

Słowa kluczowe

EN

bioeffective-linked analysis; derivatization; HPTLC; linear calibration range; quantification; TLC; triazine herbicides; videodensitometry

PL

połączone analizy bioefektywne; derywatyzacja; HPTLC; liniowy zakres kalibracji; kwantyfikacja; TLC; herbicydy triazynowe; video densytometria

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2011
• Tom: Vol. 7, No. 2
• Strony: 47--52
• Opis fizyczny: Bibliogr. 7 poz., rys., wykr.

Twórcy

autor

Broszat M.

autor

Ernst H.

autor

Spangenberg B.

• University of Offenburg, Institute of Process Engineering, Badstrasse 24, D-77652 Offenburg, Germany, Fax [phone],

Bibliografia

• Broszat, M., H. Ernst, B. Spangenberg. 2010. A simple method for quantifying triazine herbicides using thin-layer chromatography and a CCD camera. Journal of Liquid Chromatography and Related Technologies 33: 948-956.
• Jork, H., B. Roth. 1977. Vergleichende chromatographische Untersuchung bei s-Triazinen. Journal of Chromatography 144: 39-56.
• Kovác, J., M. Henselová. 1977. Detection of triazine herbicides in soil by a Hill-reaction inhibition technique after thin-layer chromatography. Journal of Chromatography 133: 420-422.
• Kovác, J., M. Kurucová, V. Bátora, J. Tekel, V. Sterniková. 1983. Chronometric technique for the quantitative analysis of some photosynthesis-inhibiting herbicides. Journal of Chromatography 280: 176-180.
• Lawrence, J.F. 1980. Simple, sensitive, and selective thin layer chromatographic technique for detecting some photosynthesis inhibiting herbicides. Journal of the Association of Official Analytical Chemists 63: 758-761.
• Sackmauerová, J., J. Kovácˇ. 1978. Thin layer chromatographic determination of triazine and urea herbicides in water by inhibition detectionmethod. Fresenius’ Zeitschrift für Analytische Chemie 292: 414-415.
• Spangenberg, B. 2006. Does the Kubelka-Munk theory describe TLC evaluations correctly? Journal of Planar Chromatography 19: 332-341.