Benzo[a]pyrene and cyclopenta[c]phenanthrene suppress expression of p53 in head kidney of rainbow trout

• Autorzy: Brzuzan P. , Woźny M. , Góra M. , Łuczyński M. K. , Jabłońska A.
• Języki publikacji: EN

Abstrakty

EN

Although p53, a protein of important tumor suppressive function, has been extensively studied in mammals, relatively little is known about the p53 pathways in lower vertebrates. Particularly, limited information exists on possible influences of environmental contaminants on the expression of the p53 gene in fish. In the current study, we assessed the effects of benzo[a]pyrene (B[a]P; potent tumor promoter) and cyclopenta[c]phenanthrene (CP[c]Ph; clastogenic agent) exposure on a 24h profile of p53 gene expression in head kidney of juvenile rainbow trout (Oncorhynchus mykiss). To analyze the p53 transcription rate, we developed protocol for the examination of both mRNA and heterogeneous nuclear (hn) RNA of the gene, using Real-Time RTPCR approach. The results show that both compounds are capable of suppressing p53 transcriptional activity within 12h of the treatment. Our finding supports the idea that structurally different PAHs may influence cell physiologic functions controlled by p53 in fish, in part, by down-regulating its RNA expression levels.

Słowa kluczowe

EN

B[a]P; CP[c]Ph; gene expression; hnRNA; rainbow trout; transcription rate

PL

B[a]P; CP[c]Ph; ekspresja genów; hnRNA; pstrąg tęczowy; poziom transkrypcji

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2011
• Tom: Vol. 7, No. 1
• Strony: 41--45
• Opis fizyczny: Bibliogr. 23 poz., rys., tab.

Twórcy

autor

Brzuzan P.

autor

Woźny M.

autor

Góra M.

autor

Łuczyński M. K.

autor

Jabłońska A.

• Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, ul. Słoneczna 45G, 10-718 Olsztyn, Poland, phone: [phone]; fax. [phone],

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