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Exposure of whitefish (Coregonus lavaretus) eggs to native or chemically spiked sediments from Lake Thun does not lead to abnormal gonad development

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Języki publikacji
EN
Abstrakty
EN
A high number of whitefish, Coregonus lavaretus, from Lake Thun, Switzerland, display gonad malformations. We tested the hypothesis that exposure to sediment-borne contaminants during the embryonic life results in the development of malformed gonads later in ontogeny. The investigated contaminants were 2,4,6-trinitrotoluene (TNT), which may leak from residues in the lake sediments as consequence of former ammunition dumping into Lake Thun, as well as sulfonated naphthalene formaldehyde condensates (SNFC), which are introduced into the lake from wastewater disposals of a nearby tunnel construction site. Experimentally, whitefish eggs were exposed during 52 days from fertilization until hatching to a) an artificial sediment (control), b) an artificial sediment spiked with TNT (0.5mg*kg-1 dry weight), c) SNFC compounds dissolved in water (3µg*l-1 of each naphthalene-1-sulphonate, naphthalene-2-sulphonate, naphthalene-1,5-disulphonate, naphthalene-2,7-disulphonate), and d) sediment from Lake Thun sampled in an ammunition dumping area. To mimic in situ exposure of the eggs to the sediment-water-interface under laboratory conditions, we developed a novel contact incubation technique. After hatching, fish were reared in tap water for three years until they reached sexual maturity, and were then examined for the presence of gonad malformations. No malformations were observed in the control, in the TNT and SNFC treatment groups. In fish incubated during the embryonic stage on Lake Thun sediment, 2 out of 117 adult males (1.7%) displayed malformed gonads, which is significantly lower than levels of gonad malformations in wild whitefish from Lake Thun (on average 29% in males, 12% in females). The results from our experiment provide no evidence that sediment contamination with TNT or SNFC compounds is a causative factor for the induction of gonad malformations in Lake Thun whitefish.
Rocznik
Strony
17--29
Opis fizyczny
Bibliogr. 35 poz., fot., wykr.
Twórcy
autor
autor
autor
autor
  • Centre for Fish and Wildlife Health, University of Bern, Länggass-Strasse 122, Post-Box 8466, CH-3001 Bern, Switzerland, Phone +41 31 631 24 41, Fax +41 31 631 24 61, helmut.segner@vetsuisse.unibe.ch
Bibliografia
  • Bernet, D., T. Wahli, C. Kueng, H. Segner. 2004. Frequent and unexplained gonadal abnormalities in whitefish (central Alpine Coregonus sp.) from an Alpine oligotrophic lake in Switzerland. Diseases of Aquatic Organisms 61: 137-148.
  • Bernet, D., A. Liedtke, D. Bittner, R.I.L. Eggen, S. Kipfer, C. Küng, C. Largiader, M. J.-F. Suter, T. Wahli, H. Segner. 2008. Gonadal malformations in whitefish from Lake Thun: defining the case and evaluating the role of EDCs. Chimia 62: 383-388.
  • Bernet, D., T. Wahli, C. Küng, H.-R. Zieri, H. Segner. Zooplankton feeding induces gonad malformations in whitefish from Lake Thun, Switzerland. In preparation.
  • Berset, J.D., U. Ochsenbein, M. Zeh. 2007. Are Lake Thun and Lake Brienz contaminated with explosive residues? Chimia 61: 532-532.
  • Berset, J.D. 2007. Sprengstoffspuren in allen drei grossen Berner Seen. GSA-Informationsbulletin: 2-7.
  • Bittner, D., D. Bernet, T. Wahli, H. Segner, C. Küng, C.R. Largiadér. 2009. How normal is abnormal? - Discriminating between malformations and natural variation in gonad morphology of European whitefish Coregonus lavaretus (L). Journal of Fish Biology 74: 1594-1614.
  • Bittner, D., A.R. Cossins, H. Segner, L. Excoffier, C.R. Largiadér. 2011. Identification of candidate genes and physiological pathways involved in gonad deformation of whitefish (Coregonus lavaretus) from Lake Thun, Switzerland. International Journal of Environmental Research and Public Health, submitted.
  • Bogdal, C., M. Naef, P. Schmid, M. Kohler, M. Zennegg, D. Bernet, M. Scheringer, K. Hungerbühler. 2009. Unexplained gonad alterations in whitefish (Coregonus spp.) from Lake Thun, Switzerland: levels of lersistent organic pollutants in different morphs. Chemosphere 74: 434-440.
  • Brannon, J.M., J.C. Pennington. 2002. Environmental Fate and Transport Process Descriptors for Explosives, US Army Corps of Engineers Report ERDC/EL TR-02-01, Available at http://el.erdc.usace.army.mil/elpubs/pdf/trel02-10.pdf
  • Brem, D., R. Gälli. 2005. Beurteilung des Einsatzes von Bauchemikalien beim Bau der NEAT am Lötschberg - Stoffflussanalyse und grobe Abschätzung der ökologischen Risiken. Short report for the Amt für Gewässerschutz und Abfallwirtschaft des Kantons Bern, Abteilung Gewässer- & Bodenschutzlabor.
  • Conder, J.M., T.W. La Point, J.A. Steevens, G.R. Lofuto. 2004. Recommendations for the assessment of TNT toxicity in sediment. Environmental Toxicology and Chemistry 23: 141-149.
  • Douglas, M.R., P.C. Brunner, L. Bernatchez. 1999. Do assemblages of Coregonus (Teleostei: Salmoniformes) in the Central Alpine region of Europe represent species flocks? Molecular Ecology 8: 589-603.
  • Della Torre, C., I. Corsi, S. Focardi, A. Arukwe. 2008. Effects of 2,4,6-trinitrotoluene (TNT) on neurosteroidgenesis in the European eel (Anguilla anguilla). Chemistry and Ecology 24: 1-7.
  • Devlin R.H., Y. Nagahama. 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208: 191-364.
  • Eisentraeger, A., G. Reifferscheid, F. Dardenne, R. Blust, A. Schofer. 2007. Hazard characterization and identification of a former ammunition site using microarrays, bioassays, and chemical analysis. Environmental Toxicology & Chemistry 26: 634-646.
  • Ek, H., G. Dave, J. Sturve, B.C. Almroth, E. Stephensen, L. Förlin, G. Birgersson. 2005. Tentative biomarkers for 2,4,6-trinitrotoluene (TNT) in fish (Oncorhynchus mykiss). Aquatic Toxicology 72: 221-230.
  • Elovitz, M.S., E.J. Weber. 1999. Sediment mediated reduction of 2,4,6-trinitrotoluene and fate of the resulting aromatic (poly)amines. Environmental Science and Technology 33: 2617-2625.
  • Fatio, V. 1890. Faune des Vertéebrées de la Suisse, Vol. V, 2e partie. Geneva: H. Georg, Libraire-éditeur.
  • Kelts, K., U. Briegel, K. Ghilardi, K. Hsu. 1986. The limnogeology-ETH coring system. Schweizerische Zeitschrift für Hydrologie 48: 104-115.
  • Kipfer, S., H. Segner, M. Wenger, T. Wahli, D. Bernet. 2009. Long term laboratory estrogen (E2) exposure of whitefish (central alpine Coregonus lavaretus) induces intersex but not the Lake Thun-typical gonad malformations. Diseases of Aquatic Organisms 84: 43-56.
  • Kirchhofer, A., P. Tschumi. 1986. Age structure and growth of coregonid fish populations in Lake Thun. Archives of Hydrobiology 22: 303-318.
  • Lotufo, G., W. Blackburn, S.J. Marlborough, J.W. Fleeger, 2010. Toxicity and bioaccumulation of TNT in marine fish in sediment exposures. Ecotoxciology and Environmental Safety 73: 1720-1227.
  • Lotufo, G. 2011. Whole-body and body-part-specific bioconcentration of explosive compounds in sheepshead minnows. Ecotoxiology and Environmental Safety 74: 301-306.
  • Milston, R.H., M.S. Fitzpatrick, A.T. Vella, S. Clements, D. Gundersen, G. Feist, T.L. Crippen, J. Leong, C.B. Schreck. 2003. Short-term exposure of chinook salmon (Oncorhynchus tshawytscha) to o,p’-DDE or DMSO during early life history stages causes long-term humoral immunosuppression. Environmental Health Perspectives 111: 1601-1607.
  • Ochsenbein, U., M. Zeh, J.D. Berset. 2008. Comparing solid phase extraction and direct injection for the analysis of ultra-trace levels of relevant explosives in lake water and tributaries using liquid chromatography-electrospray tandem mass spectrometry. Chemosphere 72: 974-980.
  • Ottinger, C.A., S.L. Kaatari. 2000. Long-term immune dysfunction in rainbow trout (Oncorhynchus mykiss) exposed as embryos to aflatoxin B1. Fish and Shellfish Immunology 10: 101-106.
  • Paden, N.E., J.A. Carr, R.J. Kendall, M. Wages, E.E. Smith. 2010. Expression of steroidogenic acute regulatory protein (StAR) in male American bullfrog (Rana catesbeiana) and preliminary evaluation of the response to TNT. Chemosphere 80: 41-45.
  • Papoulias, D.M., S.A. Villalobos, J. Meadows, D.B. Noltie, J.P. Giesy, D.E. Tillitt. 2003. In ovo exposure to o,p-DDE affects sexual development but not sexual differentiation in Japanese medaka (Oryzias latipes). Environmental Health Perspectives 111: 29-32.
  • Rufli, H. 1978. Die heutigen sympatrischen Felchenpopulationen (Coregonus spp.) des Thuner- und des Bielersees und ihre Morphologie. Schweizerische Zeitschrift für Hydrologie 40: 7-31.
  • Segner H. 2011. Reproductive and developmental toxicity in fishes. In: Gupta, R.C. (ed) Reproductive and Developmental Toxicology. Elsevier, Amsterdam, pp. 1145-1166.
  • Steinmann, P. 1950. Monographie der schweizerischen Koregonen. Schweizerische Zeitschrift für Hydrologie 12: 340-491.
  • Talmage, S.S., D.M. Opresko, C.J. Maxwell, C.J. Welsh, F.M. Cretella, P.H. Reno, F.B. Daniel. 1999. Nitroaromatic munition compounds: environmental effects and screening values. Review of Environmental Contamination and Toxicology 161: 1-156.
  • Tiedeken, J.A., J.S. Ramsdell. 2007. Embryonic exposure to domoic acid increases the susceptibility of zebrafish larvae to the chemical convulsant pentylenetetrazole. Environmental Health Perspectives 115: 1547-1552.
  • Wagler, E. 1937. Die Coregonen in den Seen des Voralpengebietes. IX. Die Systematik der Voralpen-Coregonen. International Revue Hydrobiology 35: 345-446.
  • Yoo, L.J., G.R. Lotufo, A.B. Gibson, J.A. Steevesn, J.G. Sims. 2006. Toxicity and bioaccumulation of 2,4,6-trinitrotoluene in fathead minnow (Pimephales promelas). Environmental Toxicology & Chemistry 25: 3252-3260.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-article-BAR0-0065-0003
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