Cytologic ploidy determination in fish - an example of two salmonid species

• Autorzy: Jankun M. , Kuzminski H. , Furgala-Selezniow G.
• Języki publikacji: EN

Abstrakty

EN

The method of ploidy level determination depends on the equipment, expertise of the lab or farm staff, and on the material studied (fish species, specimens' age and size, etc.). The combination of some simple techniques can be very helpful for fast, reliable and inexpensive assessment of effects of genome manipulations. Among indirect methods the nucleoli quantification seems to be the most convenient one, except that it is limited to species possessing one locus of nucleolar organizer regions (NORs) (rainbow trout as an example). Species with multilocus NORs (like brook trout) need another method, for instance sizing of erythrocytes nuclei. These techniques can be useful for identification of products resulting from new approaches in biotechnology applied to fish culture, as polyploidisation or hybridisation, for characteristics of transformation levels of organisms found in the wild or those produced due to experimental treatments.

Słowa kluczowe

EN

aquaculture; salmonid species; ploidy level determination; genome manipulation

PL

akwakultura; ryby łososiowate; poziom ploidii; manipulacje genomowe

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2007
• Tom: Vol. 3, No. 2
• Strony: 52--56
• Opis fizyczny: Bibliogr. 42 poz., rys., tab.

Twórcy

autor

Jankun M.

autor

Kuzminski H.

autor

Furgala-Selezniow G.

• Department of Ichthyology, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, ul. M. Oczapowskiego 5, Poland, phone: [phone], fax: [phone],

Bibliografia

• Anjum, R., M. Jankun. 1998. NOR-bearing chromosomal associations revealed through silver and sequential chromomycin A3 staining in the mirror carp, Cyprinus carpio L. Caryologia 51: 167-171.
• Arai, K. 2001. Genetic improvement of aquaculture finfish species by chromosome manipulation techniques in Japan. Aquaculture 197: 205-228.
• Babiak, I., S. Dobosz, K. Goryczko, H. Kuzminski, P. Woznicki. 1998. Androgenesis in rainbow trout, Oncorhynchus mykiss, using gamma irradiation and heat shock. Aquaculture Europe ‘98. Aquaculture and Water: fish culture, shellfish culture and water usage. Bordeaux, France. October 7-10, 1998.
• Benfey, T.J. 1996. Use of all-female and triploid salmonids for aquaculture in Canada. Bulletin of the Aquaculture Association of Canada 96: 6-8.
• Benfey, T.J. 1999. The physiology and behaviour of triploid fishes. Reviews in Fisheries Sciences 7: 39-67.
• Benfey, T.J., A.M. Sutterlin. 1984. The haematology of triploid landlocked Atlantic salmon Salmo salar L. Journal of Fish Biology 24: 333-338.
• Benfey, T.J., A.M. Sutterlin, R.J. Thompson. 1984. Use of erythrocyte measurement to identify triploid salmonids. Canadian Journal of Fisheries and Aquatic Sciences 41: 980-984.
• Boroń, A. 1994a. Use of erythrocyte measurements to detect natural triploids of spined loach Cobitis taenia (L.). Cytobios 78: 197-202.
• Boroń, A. 1994b. Karyotypes of diploid and triploid silver crucian carp Carassius auratus gibelio (Bloch). Cytobios 80: 117-124.
• Castro, J., A. Vinas, L. Sanchez, P. Martinez. 1996. Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta) with Ag- and CMA3-staining, and fluorescent in situ hybridization. Cytogenetics and Cell Genetics 75: 234-239.
• Chourrout, D., B. Chevassus, F. Krieg, A. Happe, G. Burger, P. Renard. 1986. Production of second generation triploid and tetraploid rainbow trout by mating tetraploid males and diploid females - potential of tetraploid fish. Theoretical and Applied Genetics 72: 193-206.
• Colihueque, N., P. Ittura, F. Estay, N.F. Diaz. 2001. Diploid chromosome number variations and sex chromosome polymorphism in five cultured strains of rainbow trout (Oncorhynchus mykiss). Aquaculture 198: 63-77.
• Dorson, M., B. Chevassus, C. Torhy. 1991. Comparative susceptibility of three species of char and of rainbow troutXchar triploid hybrids to several pathogenic salmonid viruses. Diseases of Aquatic Organisms 11: 217-224.
• Flajshans, M., P. Rab, S. Dobosz. 1992. Frequency analyses of active NORs in nuclei of artificially induced triploid fishes. Theoretical and Applied Genetics 85: 68-72.
• Foresti, F. 2000. Biotechnology and fish culture. Hydrobiologia 420: 45-47.
• Foresti, F., C. Oliveira, P.M. Galetti, Jr., L.F. Almeida-Toledo. 1993. Synaptonemal complex analysis in spermatocytes of tilapia, Oreochromis niloticus (Pisces, Cichlidae). Genome 36: 1124-1128.
• Foresti, F., C. Oliveira, E.D. Carvalho. 1994. Ploidy evaluation in the pacu fish, Piaractus mesopotamicus (Pisces, Characiformes): techniques and comments. Revista Brasileira de Biologia 54: 31-37.
• Fujiwara, A., S. Abe, E. Yamaha, F. Yamazaki, M.C. Yoshida. 1998. Chromosomal localization and heterochromatin association of ribosomal regions in salmonid fishes. Chromosome Research 6: 463-471.
• Galetti, P.M., E.M. Rasch. 1993. Chromosome studies in Poecilia latipunctata with NOR polymorphism as shown by silver nitrate and chromomycin A3 (Teleostei: Poeciliidae). Ichthyological Exploration of Freshwaters 4: 269-277.
• Hardie, D.C., T.R. Gregory, P.D.N. Hebert. 2002. From pixels to picograms: A beginners' guide to genome quantification by Feulgen image analysis densitometry. Journal of Histochemistry and Cytochemistry 50: 735-749.
• Harrella, R.M., W. Van Heukelema, J.H. Kerbyb. 1998. A comparison of triploid induction validation techniques. The Progressive Fish-Culturist 60: 221-226.
• Howell, W.M., D.A. Black. 1980. Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014-1015.
• Kucharczyk, D., M. Jankun, M. Luczynski. 1997. Ploidy level determination in genetically manipulated bream, Abramis brama L., based on the number of active nucleoli per cell. Journal of Applied Aquaculture 7: 13-21.
• Maxime, V. 2008. The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish and Fisheries 9: 67-78.
• Mellink, C.H.M., A.A. Bosma, N.A. De Haan, A.A. MacDonald. 1992. Numerical variation of nucleolar organizer regions after silver staining in domestic and wild Suidae (Mammalia). Animal Genetics 23: 231-239.
• Ocalewicz, K. 2002. Cytogenetic markers for X chromosome in karyotype of rainbow trout from Rutki strain. Folia biologia (Krakow) 50: 10-14.
• Ocalewicz, K, M. Jankun, M. Luczynski. 2007. Chromosome set manipulations and cytogenetic characteristic of polyploid, gynogenetic and androgenetic fish strains. In: Fish Cytogenetics (ed. E. Pisano, C. Ozouf-Costaz, F. Foresti, B. G. Kapoor), pp. 289-332. Science Publishers, New Hampshire, USA.
• Ozouf-Costaz, C., F. Foresti. 1992. Fish cytogenetic research: advances, applications and perspectives. Netherlands Journal of Zoology 42: 277-290.
• Ozouf-Costaz, C., G.G. Teugels, M. Legrande. 1990. Karyological analysis of three strains of the African Clariidae catfish Clarias gariepinus used in aquaculture. Aquaculture 88: 271-277.
• Pandian, T.J., R. Koteeswaran. 1998. Ploidy induction and sex control in fish. Hydrobiologia 384: 167-243.
• Pardo, B.G., C. Bouza, J. Castro, P. Martinez, L. Sanchez. 2001. Localization of ribosomal genes in Pleuronectiformes using Ag-, CMA3-banding and in situ hybridization. Heredity 86: 531-536.
• Phillips, R.B., P.E. Ihssen. 1985. Chromosome banding in salmonid fish: nucleolar organizer regions in Salmo and Salvelinus. Canadian Journal of Genetics and Cytology 27: 433-440.
• Phillips, R.B., K.D. Zajicek, P.E. Ihssen, O. Johnson. 1986. Application of silver staining to the identification of triploid fish cells. Aquaculture 54: 313-319.
• Reeder, R.H. 1990. rRNA synthesis in the nucleolus. Trends in Genetics 6: 390-395.
• Schmid, M., W. Feichtinger, R. Weimer, C. Mais, F. Bolaños, P. León. 1995. Chromosome banding in Amphibia. XXI. Inversion polymorphism and multiple nucleolus organizer regions in Agalychnis callidryas (Anura, Hylidae). Cytogenetics and Cell Genetics 69: 18-26.
• Ueda, T., R. Sato, J. Kobayashi. 1988. Silver banded karyotypes of the rainbow trout and the brook trout and their hybrids: disappearance in allotriploids of Ag-NORs originated from the brook trout. Japanese Journal of Genetics 63: 219-226.
• Wlasow, T., H. Kuzminski, P. Woznicki, E. Ziomek. 2004. Blood cells alteration in triploid brook trout Salvelinus fontinalis (Mitchill). Acta Veterinaria (Brno) 73: 25-29.
• Wolf, K. 1988. Fish Viruses and Fish Viral Diseases. 476 p. Comstock Publishing Association, Cornell University Press, Ithaca, USA.
• Woznicki, P., M. Jankun. 1994. Chromosome polymorphism of Atlantic salmon (Salmo salar) from the River Dzwina, Baltic Sea basin: arm lenght and NOR location variation of the eighth chromosome. Canadian Journal of Zoology 72: 364-367.
• Woznicki, P., H. Kuzminski. 2002. Chromosome number and erythrocyte nuclei length in triploid brook trout (Salvelinus fontinalis). Caryologia 55: 295-298.
• Woznicki, P., L. Sanchez, P. Martinez, B.G. Pardo, M. Jankun. 2000. A population analysis of the structure and variability of NOR in Salmo trutta by Ag, CMA3 and ISH. Genetica 108: 113-118.
• Yokote, M. 1982. Vascular system. In: An Atlas of Fish Histology Normal and Pathological Features (ed. T. Hibiya), pp. 58-73. Gustav Fischer Verlag, Stuttgart.