Influence of photodegradation on mutagenic activity of aquatic solution of chlorophenols

• Autorzy: Czaplicka M. , Mielżyńska D.
• Języki publikacji: EN

Abstrakty

EN

The paper presents the effect of UV radiation of 254 nmwavelength on the mutagenic activity of aquatic solutions of 3-chlorophenol (3-CP), 2,4-dichlorophenol (PCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP). Mutagenicity studies were carried out on two Salmonella strains: TA98 and TA100, with and without metabolic activation by S9 mix. Stable intermediate products of photodegradation have been identified using the GC/MS method. Chlorophenols in doses below 0.1µg were strongly mutagenic towards both strains: TA98 and TA100. The mutagenic properties of aquatic solutions of chlorophenols for both strains decreased as follows: PCP>=2,4-DCP>2,4,6-TCP>3-CP. Aquatic solutions of three chlorophenols after their photodegradation were non-mutagenic for both bacterial strains, except for 2,4-dichlorophenol. In the case of this compound, the products of its decomposition were more mutagenic for the TA100 strain. No significant metabolic activation by S9 mix was observed which suggests a direct mutagenic impact of chlorophenols on the tested Salmonella strains.

Słowa kluczowe

EN

chlorophenols; mutagenic activity; photodegradation; Salmonella assay

PL

chlorofenol; aktywność mutagenna; fotodegradacja; test Salmonella

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2007
• Tom: Vol. 3, No. 1
• Strony: 30--36
• Opis fizyczny: Bibliogr. 29 poz., tab., wykr.

Twórcy

autor

Czaplicka M.

autor

Mielżyńska D.

• Institute of Non-Ferrous Metals, Gliwice, Sowińskiego 5, Poland,

Bibliografia

• Bernstein, L., J. Kaldor, J. McCann, M.C. Pike. 1982. An empirical approach to the statistical analysis of mutagenesis data from Salmonella test. Mutation Research 97: 267-272.
• Bradford, M.M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-253.
• Czaplicka, M. 2001. Determination of phenols and chlorophenols in bottom sedmiments. Chromatographia 53: 470-475.
• Czaplicka, M. 2004. Sources and transformation of chlorophenols in the natural environment. The Science of the Total Environment 322: 21-39.
• DeMarini, D.M., H.G. Brooks, D.G. Parkes, Jr. 1990. Induction of prophage lambda by chlorophenols. Environmental and Molecular Mutagenesis 15: 1-9.
• Environmental Health Criteria 93. 1987. Chlorophenols Other Than Pentachlorophenol. WHO, Geneva.
• Fahrig,R., C. Nilsson, C. Rappe. 1978.Genetic activity of chlorophenols and chlorophenol impurities. Environmental Science Research 12: 325-338.
• Galloway, S.M., M.J. Armstrong, C. Reuben, S. Colman, B. Brown, C. Cannon, A.D. Bloom, F. Nakamura,M. Ahmed, S.Duk, J. Rimpo, B.H. Margolin, M.A. Resnick, B. Anderson, E. Zeiger. 1987. Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environmental and Molecular Mutagenesis 10: 1-175.
• Hattula, M.L., J. Knuutinen. 1985. Mutagenesis of mammalian cells in culture by chlorophenols, chlorocatechols and chloroguaiacols. Chemosphere 14: 1617-1625.
• Haworth, S., T. Lawlor, K. Mortelmans, W. Speck, E. Zeiger. 1983. Salmonella mutagenicity test results for 250 chemicals. Environmental Mutagenesis (Suppl. 1) 3: 136-142.
• IARC. 1987 (update 1999). Monographs on the Evaluation of Carcinogenic Risks to Humans. Vol. 41. Some Halogenated Hydrocarbons and Pesticide Exposures. Lyon.
• Jansson, K., V. Jansson. 1992. Genotoxicity of 2,4,6-trichlorophenol in V79 Chinese hamster cells. Mutation Research 280: 175-179.
• Kinae, N., T. Hashizume, T. Makita. 1981. Studies on the toxicity of pulp and paper mill effluents. I. Mutagenicity. Water Research 15: 17-24.
• Maron, D.M., B.N. Ames. 1983. Revised methods for the Salmonella mutagenicity test. Mutation Research 113: 173-215.
• McConnell, E.E. 1989. NTP Technical report on the toxicology and carcinogenesis studies of pentachlorophenol (Cas No. 87-86-5) in B6C3F1 mice (feed studies). National Toxicology Program Technical Report NTP TR 1989, 349: 5-11 and 83-86.
• McGregor, D.B., A. Brown, P. Cattanach. 1988. Responses of the LS 178Ytk+/tk- mouse lymphoma cell forward mutation assay. III. 72 Coded chemicals. Environmental and Molecular Mutagenesis 12: 85-154.
• Nishimura, N., H. Oshima. 1983. Mutagenicity of pentachlorophenol, dinitro-o-cresol and related compounds. Japanese Journal of Industrial Health 25: 510-511.
• Nishimura, N., H. Nishimura, H. Oshima. 1982. Survey on mutagenicity of pesticides by the Salmonella/microsome test. Journal of the Aichi Medical University Association 10: 305-312.
• Ohara P., M. Torabinejad, J. D. Kettering. 1993. Antibacterial effects of various endodontic medicaments on selected anaerobic bacteria. Journal of Endodontics 19: 498-500.
• One, Y., I. Somiya, T. Kawaguchi. 1992. Genotoxic evaluation on aromatic organochlorine compounds by using umu test. Water Science Technology 26: 61-69.
• Onfelt, A. 1987. Spindle disturbances in mammalian cells. III. Toxicity, c-mitosis and aneuploidy with 22 different compounds: Specific and unspecific mechanisms. Mutatation Research 182: 135-154.
• Probst, G.S., R.E. McMahon, L.E. Hill. 1981. Chemically-induced unscheduled DNA synthesis in primary rat hepatocyte cultures: A comparison with bacterial mutagenicity using 218 compounds. Environmental Mutagenesis 3: 11-15.
• Rapson, W.H., M.A. Nazar, V.V. Bulsky. 1980. Mutagenicity produced by aqueous chlorination of organic compounds. Bulletin of Environmental Contamination and Toxicology 24: 590-596.
• Rasanen, L., M.L. Hattula, A.U. Arstila. 1977. Mutagenicity of MCPA and its soil metabolites, chlorinated phenols, catechols and some widely used slimicides in Finland. Bulletin of Environmental Contamination and Toxicology 18: 565-571.
• Saito, H., T. Shigeoka. 1994. Comparative cytotoxicity of chlorophenols to cultured fish cells. Environmental Toxicology and Chemistry 13: 1649-1650.
• Seiler, J.P. 1991. Pentachlorophenol. Mutation Research 257: 27-47.
• Seuferer, S.L., I,-I.D. Braymer, J.J. Dunn. 1979. Metabolism of diflubenzuron by soil microorganisms and mutagenicity of the metabolites. Pesticide Biochemistry and Physiology 10: 174-180.
• Strobe, K., T. Grummt. 1987. Aliphatic and aromatic halocarbons as potential mutagens in drinking water. Part II. Chlorinated phenols. Toxicological and Environmental Chemistry 14: 143-156.
• Vollmuth, S., A. Zajc, R. Niessner. 1994. Formation of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans during the photolysis of pentachlorophenol-containing water. Environmental Science & Technology 28: 1145-1149.