Influence of oxygen O2 on microwave saturation of EPR lines of plants carbonized at 650°c and potential application in medicine

• Autorzy: Pilawa B. , Bartłomiejczyk S. , Krzesińska M. , Pusz S. , Zachariasz J. , Wałach W.
• Języki publikacji: EN

Abstrakty

EN

Electron paramagnetic resonance spectroscopy (EPR) was used to examination of spin-lattice relaxation in vascular plants carbonized at 650°C. Application of EPR method of continuous microwave signal saturation in medicine was proposed. The first derivative EPR spectra of pyrolysed bamboo and yucca were measured for samples in air and in vacuum. Influence of microwave power in the range 0.7-100 mW on amplitudes of EPR lines were evaluated. It was stated that amplitudes of the studied carbon materials increase with increasing of microwave power in the studied range. Saturation of EPR lines was not observed, so fast spin-lattice relaxation processes exist in the analyzed materials. Changes of EPR amplitudes for samples in air were slower than for evacuated samples. This effect may be used for determination of oxygen contents in the biological cells cultures and for analysis of optimal parameters of photodynamic therapy of cancer.

Słowa kluczowe

EN

carbonized plants; electron paramagnetic resonance; EPR; microwave saturation; paramagnetic centers; oximetry

PL

karbonizacja roślin; elektroniczny rezonans paramagnetyczny; EPR; nasycenie mikrofalowe; centra paramagnetyczne; oksymetria

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2008
• Tom: Vol. 11, no. 73
• Strony: 9--11
• Opis fizyczny: Bibliogr. 14 poz., rys., wykr.

Twórcy

autor

Pilawa B.

• Centre of Polymer and Carbon Materials, Polish Academy of Science, Marii Curie-Skłodowskiej 34, 41-819 Zabrze, Poland

autor

Bartłomiejczyk S.

• Centre of Polymer and Carbon Materials, Polish Academy of Science, Marii Curie-Skłodowskiej 34, 41-819 Zabrze, Poland

autor

Krzesińska M.

• Centre of Polymer and Carbon Materials, Polish Academy of Science, Marii Curie-Skłodowskiej 34, 41-819 Zabrze, Poland

autor

Pusz S.

• Centre of Polymer and Carbon Materials, Polish Academy of Science, Marii Curie-Skłodowskiej 34, 41-819 Zabrze, Poland

autor

Zachariasz J.

• Centre of Polymer and Carbon Materials, Polish Academy of Science, Marii Curie-Skłodowskiej 34, 41-819 Zabrze, Poland

autor

Wałach W.

• Centre of Polymer and Carbon Materials, Polish Academy of Science, Marii Curie-Skłodowskiej 34, 41-819 Zabrze, Poland

Bibliografia

• [1] Dunn J. F., Swartz H. M.: In vivo electron paramagnetic resonance oximetry with particulate materials, Methods 30(2003) 159-166.
• [2] Santini M. T., Cametti C, Straface E., Floridi A., Flamma F., Paradisi S., Malorni W.: The new EPR molecular oxygen probe fusinite is not toxic to cells, Biochimica et Biophysica Acta 1379(1998) 161-170.
• [3] Ligeza A. Tikhonov A. N. Subczynski W. K.: In situ measurements of oxygen production and consumption using paramagnetic fusinite particles injected into a bean leaf, Biochimica et Biophysica Acta 1319(1997)133-1337.
• [4] Atsarkin V. A., Demidov V. V., Vasneva G. A., Dzheparov F. S., Ceroke P. J., Odintsov B. M., Clarkson R. B.: Mechanism of oxygen response in carbon-based sensors, Journal of Magnetic Resonance 149(2001)85-89.
• [5] Manivannan A., Yanagi H., IIangovan G., Kuppusamy P: Lithium naphthalocyanine as a new molecular radical probe for electron paramagnetic resonance oximetry, Journal of Magnetism and Magnetic Materials 223(2001) L131-L135.
• [6] Pilawa B., Latocha M., Kościelniak M., Pietrzak R., Wachowska H.: Oxygen effects in tumor cells during photodynamic therapy, Polish Journal of Environmental Studies 15(2006) 160-162.
• [7] Stankowski J., Hilczer W.: Pierwszy krok ku radiospektroskopii rezonansów magnetycznych, Ośrodek Wydawnictw Naukowych, Poznań 1994.
• [8] Eaton G. R., Eaton S. S., Salikhov K. M. (Eds.): Foundations of Modern EPR, World Scientific Publishing Co., Singapore, New Jersey, London, Hong Kong 1998.
• [9] Pilawa B., Trzebicka B., Więckowski A. B„ Hanak B., Komorek J., Pusz S.: EPR spectra of exinite, vitrinite and inertinite. Influence of microwave saturation and sample evacuation, Erdol & Kohle Erdgas Petrochemie - Hydrocarbon Technology 44(1991) 421-425.
• [10] Pilawa B., Więckowski A. B.: Comparative e.p.r. analysis of interactions between macerals and atmospheric oxygen, Fuel 76 (1997) 1173-1177.
• [11] Krzesińska M., Pilawa B., Pusz S., Ng J.: Biologiczne prekursory dla tzw. „drewnianych" ceramik (woodceramics) - otrzymywanie i właściwości, Inżynieria Materiałowa 1(149) (2006) 32-36.
• [12] Krzesińska M., Pilawa B., Pusz S., Ng J.: Physical characteristics of carbon materials derived from pyrolysed vascular plants, Biomass & Bioenergy 30 (2006) 166-176.
• [13] Graczyk A.: Fotodynamiczna metoda rozpoznawania i leczenia nowotworów, Dom Wydawniczy Bellona, Warszawa 1999.
• [14] Podbielska H., Sieroń A., Stręk W.: Diagnostyka i terapia fotodynamiczna, Wydawnictwo Medyczne Urban & Partner, Wrocław 2004.