Conditions of photodynamic therapy of tumor cells examined by carbonized coal and EPR spectroscopy

• Autorzy: Latocha M. , Pilawa B. , Pietrzak R. , Nowicki P. , Wachowska H.
• Języki publikacji: EN

Abstrakty

EN

Coal samples carbonized at 600°C and TEMPO probes we applied in oximetry. EPR spectra of medium-ranked coal carbonized at 400, 500, and 700 °C were not sensitive to oxygen. Oxygen effects during photodynamic therapy of tumor cells were tested. Oximetric probes were used to examination of singlet oxygen O2 formation in melanotic CRL-1424 tumor cells irradiated by laser (500 mW, Λ = 662 nm) at the presence of photolon as the photosensitizer. Tumor cells were irradiated during 7, 15, and 30 minutes. Changes in EPR spectra of coal probe and TEMPO after excitation of oxygen O2 from triplet ground state (S=1) to diamagnetic singlet (S=0) state were analysed. Measurements of EPR spectra of coal carbonized at 60O°C and TEMPO in: control cell culture, irradiated cells, and cells irradiated at the presence of photolon, were done. After PDT intensity of EPR lines of the used oximetric probes considerably increases. It was proved that the strongest formation of singlet oxygen in the studied cells appears after 15 minutes of laser irradiation.

Słowa kluczowe

EN

paramagnetic centers; PDT; oximetric probes; EPR

PL

centra paramagnetyczne; PDT; próby oksymetryczne; EPR

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2008
• Tom: Vol. 11, no. 73
• Strony: 6--8
• Opis fizyczny: Bibliogr. 14 poz., rys., wykr., tab.

Twórcy

autor

Latocha M.

• Department of Cell Biology, School of Pharmacy, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland

autor

Pilawa B.

• Department of Biophysics, School of Pharmacy, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland

autor

Pietrzak R.

• Laboratory of Coal Chemistry and Technology, Institute of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland

autor

Nowicki P.

• Laboratory of Coal Chemistry and Technology, Institute of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland

autor

Wachowska H.

• Laboratory of Coal Chemistry and Technology, Institute of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland

Bibliografia

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