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Coal samples carbonized at 600°C and TEMPO probes we applied in oximetry. EPR spectra of medium-ranked coal carbonized at 400, 500, and 700 °C were not sensitive to oxygen. Oxygen effects during photodynamic therapy of tumor cells were tested. Oximetric probes were used to examination of singlet oxygen O2 formation in melanotic CRL-1424 tumor cells irradiated by laser (500 mW, Λ = 662 nm) at the presence of photolon as the photosensitizer. Tumor cells were irradiated during 7, 15, and 30 minutes. Changes in EPR spectra of coal probe and TEMPO after excitation of oxygen O2 from triplet ground state (S=1) to diamagnetic singlet (S=0) state were analysed. Measurements of EPR spectra of coal carbonized at 60O°C and TEMPO in: control cell culture, irradiated cells, and cells irradiated at the presence of photolon, were done. After PDT intensity of EPR lines of the used oximetric probes considerably increases. It was proved that the strongest formation of singlet oxygen in the studied cells appears after 15 minutes of laser irradiation.
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Tom
Strony
6--8
Opis fizyczny
Bibliogr. 14 poz., rys., wykr., tab.
Twórcy
autor
- Department of Cell Biology, School of Pharmacy, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland
autor
- Department of Biophysics, School of Pharmacy, Medical University of Silesia, Jedności 8, 41-200 Sosnowiec, Poland
autor
- Laboratory of Coal Chemistry and Technology, Institute of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland
autor
- Laboratory of Coal Chemistry and Technology, Institute of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland
autor
- Laboratory of Coal Chemistry and Technology, Institute of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60-780 Poznań, Poland
Bibliografia
- [1] Uzdensky A., Dergacheva O.Y,Zhavoronkova A.A, Reshetnikov A.V, Ponomarev G.V.: Photodynamic effect of novel chlorine (6) derivatives on a single cell. Life Science 74 (2004) 2185-97.
- [2] Latocha M., Pilawa B., Kuśmierz D., Zielińska A., Nawrocka D.: Changes in free radicals system on IMR-90 and C-32 cells during photodynamic therapy. Polish Journal of Environmental Studies 15 (2006) 154-156.
- [3] Pilawa B., Latocha M., Kościelniak M., Pietrzak R., Wachowska H.: Oxygen effects in tumor cells during photodynamic therapy. Polish Journal of Environmental Studies 15 (2006) 160-162.
- [4] Pilawa B.,Trzebicka B., Więckowski A. B., Hanak B., Komorek J., Pusz S.: EPR Spectra of Exinite, Vitrinite and Inertinite. Influence of Microwave Saturation and Sample Evacuation. Erdol & Kohle Erdgas Petrochemie - Hydrocarbon Technology 44 (1991) 421-425.
- [5] Pilawa B., Więckowski A.B.: Comparative EPR analysis of interactions between macerals and atmospheric oxygen. Fuel 76 (1997) 1173-1177.
- [6] Jordan B.F., Baudelet Ch., Gallez B.: Carbon-centered radicals as oxygen sensors for in vivo electron paramagnetic resonance: screening for an optimal probe among commercially available charcoals. MAGMA Magnetic Resonance Materials in Physics Biology and Medicine 7 (1998) 121-129.
- [7] Santini M.T.: Corrigendum to 'The new EPR molecular oxygen probe fusinite is not toxic to cells'. Biochimica et Biophysica Acta 1379(1998)161-170.
- [8] Diakova G., Bryant R.G.: The aqueous reference for ESR oximetry Journal of Magnetic Resonance 178 (2006) 329-333.
- [9] Liu K.J., Miyake M., James P.E., Swartz H.M.: Separation and enrichment of the active component of the carbon based paramagnetic materials for use in EPR oximetry. Journal of Magnetic Resonance 133 (1998) 291-298.
- [10] Mizoguchi K., Kachi N., Sakamoto H., Yoshioka K., Masubuchi S., Kazama S.: The effect of oxygen on the ESR linewidth in polypyrrole doped by PF6. Solid State Communications 105 (1998) 81 -84.
- [11] IIangovan G., Zweier J.L., Kuppusamy P: Mechanism of oxygen-induced EPR line-broadening in lithium phthalocyanine microcrystals Journal of Magnetic Resonance 170 (2004) 42-48.
- [12] Venkataraman S., Martin S.M., Schafer F.Q., Buettner G.R.: Detailed methods for the quantification of nitric oxide in aqueous solutions using either an oxygen monitor or EPR. Free Radical in Biology and Medicine 29 (2000) 580-585.
- [13] Ligeza A., Tikhonov A.N., Subczynski W.K.: In situ measurements of oxygen production and consumption using paramagnetic fusinite particles injected into a bean leaf. Biochimica et Biophysica Acta. Bioenergetics 1319 (1997) 133-137.
- [14] He Y-Y, Jlang L-L.: Synthesis and EPR Investigations of new aminated hypocrellin derivatives. Free Radical in Biology and Medicine 28(2000)1642-1651.
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Bibliografia
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