Adhesion and proliferation of vascular smooth muscle cells on polylactide-polyethylene oxide copolymers with different content and length of polyethylene oxide chains

• Autorzy: Filova E. , Bacakova L. , Lisa V. , Kubies D. , Machova L. , Lapcikova M. , Rypacek F.
• Języki publikacji: EN

Abstrakty

EN

We evaluated antiadhesive effects of polymer surfaces prepared from PDLLA-PEO copolymers using PEO with a different molecular weight and different PEO content in comparison with the native poly(L-lactide) (PLLA) surface. All PDLLA-PEO copolymers significantly decreased number of initially adhered cells (by 23-55% in comparison with pure PLLA) as well as spreading area 24 hours after seeding (by 39-79%). Cell proliferation, estimated by cell number on the 6 day after seeding and bromodeoxyuridine (BrdU) labeling index, was significantly lower on PEO-containing copolymers (by 58-96% and 21-35% respectively) compared to pure PLLA surface. Immunofluorescence staining of vinculin showed that the ability of VSMC to form local adhesion plaques was markedly reduced on surfaces with the highest content of PEO (33 and 44%). Thus, these copolymers are promising for creation of surfaces preventing uncontrolled adsorption of proteins and adhesion of cells. Consecutively, binding of defined ligands for cell adhesion receptors would enable to control cell behaviour on these materials, which could be used for construction of vascular prostheses.

Słowa kluczowe

EN

vascular smooth muscle cells; copolymers

PL

kopolimery; biomateriały

• Wydawca: Polskie Stowarzyszenie Biomateriałów
• Czasopismo: Inżynieria Biomateriałów
• Rocznik: 2004
• Tom: R. 7, nr 38-42
• Strony: 19--21
• Opis fizyczny: Bibliogr. 20 poz., rys., wykr.

Twórcy

autor

Filova E.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 00, Prague 4-KRC, Czech Republic

autor

Bacakova L.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 00, Prague 4-KRC, Czech Republic

autor

Lisa V.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 00, Prague 4-KRC, Czech Republic

autor

Kubies D.

• Centre for Cell Therapy and Tissue Repair,2nd Faculty of Medicine, V úvalu 84, Prague 5, Czech Republic

autor

Machova L.

• Institute of Macromolecular Chemistry Academy of Sciences oft he Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic

autor

Lapcikova M.

• Institute of Macromolecular Chemistry Academy of Sciences oft he Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic

autor

Rypacek F.

• Institute of Macromolecular Chemistry Academy of Sciences oft he Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 00, Prague 4-KRC, Czech Republic

Bibliografia

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