Biological effects of polymers modified with carbon nanotubes on human osteoblast-like MG 63 cells

• Autorzy: Grausova L. , Bacakova L. , Frączek A. , Błażewicz S. , Błażewicz M. , Pepka M.
• Języki publikacji: EN

Abstrakty

EN

The tested materials were represented by a pure terpolymer of polytetrafluorethylene-polyvinyldifluoride-polypropylene (PTFE-PVDF-PP), pure polysulphone (PSU) and PSU modified with single- or multiwalled carbon nanotubes in concentrations of 0.5, 1 or 2 wt%. As control samples, a polystyrene cell culture dish and microscopic glass coverslips were used. The number and viability of human osteoblast-like MG 63 cells in cultures on these materials was detected with a Cell Viability Analyzer (Vi-CELL XR, Beckman Coulter) on 1, 3 and 7 days after seeding. On all tested samples, the cell number was similar or lower than that detected on the control polystyrene dishes. The cell viability on day 1 after seeding was relatively low on PTFE-PVDF-PP and some nanotube-containing samples, ranging from 10 to 100% of living cells, but on day 7 after seeding, it reached at least 90% on all tested samples. The cell spreading area was detected in cells after immunocytochemical staining of beta-actin on day 3 after seeding. In nanotube-containing samples, especially those with multi-walled nanotubes, this area was similar or even larger than that on the control materials. The beta-actin cytoskeleton was well developed in cells on all nanotube-containing materials and similar to that in cells on control surfaces. Thus, it can be concluded that nanotube-containing PSU supports the adhesion and growth of osteoblast-like cells and could be used for construction of bone implants in which the anchorage in the surrounding bone tissue is desirable.

Słowa kluczowe

EN

carbon nanoparticles; carbon-polymer composites; bone cells; adhesion; proliferation; beta-actin

PL

adhezja; nonocząstki węglanowe; osteocyt; rozmnażanie wegetatywne

• Wydawca: Polskie Stowarzyszenie Biomateriałów
• Czasopismo: Inżynieria Biomateriałów
• Rocznik: 2006
• Tom: R. 9, nr 58-60
• Strony: 14--16
• Opis fizyczny: Bibliogr. 5 poz., rys., wykr.

Twórcy

autor

Grausova L.

• Institute of Physiology. Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Bacakova L.

• Institute of Physiology. Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Frączek A.

• University of Science and Technology, AGH, Mickiewicza 30/A3, 30-059 Kraków, Poland

autor

Błażewicz S.

• University of Science and Technology, AGH, Mickiewicza 30/A3, 30-059 Kraków, Poland

autor

Błażewicz M.

• University of Science and Technology, AGH, Mickiewicza 30/A3, 30-059 Kraków, Poland

autor

Pepka M.

• Nanocraft Inc, P.O. Box 3429, Renton, WA 98056, U.S.A.

Bibliografia

• [1] Price RL, Ellison K., Haberstroh KM, Webster TJ: J. Biomed. Mater. Res. 70A: 129-138, 2004.
• [2] Webster TJ, Ergun C., Doremus RH, Siegel RW, Bizios R.: J. Biomed. Mater. Res. 51, 475-483, 2000.
• [3] Tan J., Saltzman WM: Biomaterials 25: 3593-3601, 2004.
• [4] Bacakova L., Mares V., Lisa V., Svorcik V.: Biomaterials 21: 1173-1179, 2000.
• [5] Bacakova L., Grausova L., Vacik J., Jungova I.: Inżynieria Biomateriałów (Engineering of Biomaterials), VIII (47-53): 3-6, 2005.