Human endothelium on vascular prostheses modified by extracellular matrix proteins in a flow experiment

• Autorzy: Chlupac J. , Filova E. , Riedel T. , Brynda E. , Remy-Zolghadri M. , Bareille R. , Fernandez P. , Daculsi R. , Bordenave L. , Bacakova L.
• Języki publikacji: EN

Abstrakty

EN

Artificial vascular prostheses are used for bypass surgery. Thrombogenicity often cause graft occlusion. Targeted surface alterations including cell seeding may improve the haemocompatibility. Knitted commercial tubular PET (polyethylene terephtalate) vascular prostheses with collagen impregnation were modified by adsorption of laminin (LM) or coating with fibrin network (FB) on the luminal surface. Human endothelial cells were harvested, cultured and seeded at the density of 150x10\3 cells/cm square on all grafts. The cell lining was continuously visualized and quantified. The retention was 21%, 37% and only 2% of the seeding density on the unmodified (UM), LM- and FB-coated grafts, respectively. These seeded prostheses were exposed to a laminar shear stress (SS) 15 dynes/cm square for 40 minutes (UM, LM, FB) and 120 min (UM, LM) in a chamber simulating blood circulation. The SS was excluded in static (ST) control grafts. After 40 min-SS the cell numbers were78%, 27% and 72% for the UM, LM and FB prosthesis compared to the ST. The cell densities were 61% and 57% on the UM and LM after 120 min-SS. To conclude, the endothelium formed a confluent layer whereas laminin immobilization improved endothelial adhesion but not the flow resistance. Reverse effect was observed on fibrin coating.

Słowa kluczowe

EN

endothelial cells; vascular prosthesis; laminin; fibrin; adhesion; shear stress; collagen

PL

adhezja; proteza naczyniowa; kolagen; fibryna; naprężenie styczne

• Wydawca: Polskie Stowarzyszenie Biomateriałów
• Czasopismo: Inżynieria Biomateriałów
• Rocznik: 2006
• Tom: R. 9, nr 58-60
• Strony: 10--13
• Opis fizyczny: Bibliogr. 10 poz., rys., wykr.

Twórcy

autor

Chlupac J.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Filova E.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Riedel T.

• Institute of Macromolecular Chemistry, Academy of Sciences of The Czech Republic, Heyerovsky Sq. 2, 162 06 Prague-6, Czech Republic

autor

Brynda E.

• Institute of Macromolecular Chemistry, Academy of Sciences of The Czech Republic, Heyerovsky Sq. 2, 162 06 Prague-6, Czech Republic

autor

Remy-Zolghadri M.

• Inserm U577, Universite Victor Segalen, 146 Rue Leo Saignant, Bordeaux 33076, France

autor

Bareille R.

• Inserm U577, Universite Victor Segalen, 146 Rue Leo Saignant, Bordeaux 33076, France

autor

Fernandez P.

• Inserm U577, Universite Victor Segalen, 146 Rue Leo Saignant, Bordeaux 33076, France

autor

Daculsi R.

• Inserm U577, Universite Victor Segalen, 146 Rue Leo Saignant, Bordeaux 33076, France

autor

Bordenave L.

• Inserm U577, Universite Victor Segalen, 146 Rue Leo Saignant, Bordeaux 33076, France

autor

Bacakova L.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 20 Prague 4-Krc, Czech Republic

Bibliografia

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• [9] Traub O., Berk BC: Arterioscler. Tromb, Vasc. Biol. 18: 677-685, 1998.
• [10] Gourevitch D., Jones CE, Crocker J., Goldman M.: Biomaterials Vol. 9 January, 1988.