Adhesion and growth of human osteoblast-like cells on aliphatic polyesters with different chemical composition, surface roughness and modification with hydroxyapatite

• Autorzy: Vagaska B. , Bacakova L. , Pamuła E. , Lisa V. , Dobrzyński P.
• Języki publikacji: EN

Abstrakty

EN

In this study we have investigated the effect of three groups of polymeric foils on the behavior of MG 63 osteoblast-like cells. These included (1) poly(L-lactide) (PLLA) compared with newly synthesized copolymer of L-lactide and trimethylene carbonate (PLTMC 50:50), (2) three samples made of glycolide and epsylon-caprolactone copolymer (PGCap) with different surface roughness and topography, and finally (3) copolymer of glycolide with L-lactide (PGLA) compared with its modification with hyroxyapatite deposits. On the 1st and 4th day of cultivation the cell number on all of the samples was lower than on control polystyrene culture dish. However, on day 8 after seeding, the values on the tested samples caught up with the control polystyrene. In the first group the cell number of PLTMC was higher than on polystyrene or PLLA. In the second group, the number of cells on PGCap samples of the lower surface roughness (RRMS 130 and 180 nm) was significantly higher than that on the control polystyrene, whereas on the PGCap samples with the rounghness in micrometers, it was comparable to the value on the polystyrene. Moreover, the surface roughness influenced the cell adhesion area. The cells on the sample with the highest roughness index were roundly shaped and their adhesion area was significantly lower, because the cells were restricted in their spreading by the surface structure of the material. In the last group, the number of cells on day 8 on the polymer with hydroxyapatite deposits was significantly higher than on standard tissue culture polystyrene dish, as well as on unmodified PGLA foil, which suggested that hydroxyapatite supports cell proliferation.

Słowa kluczowe

EN

glycolide; L-lactice; epsylon-caprolactone; trimethylene carbonate; polyethylene glycol; hydroxyapatite; wettability; roughness; bone cells; cell adhesion; cell proliferation

PL

osteocyt; glikolid; L-laktyd; epsilon-kaprolaktam; trimetylen; trimetylen węglanowy; hydroksyapatyt; zwilżalność; chropowatość powierzchni; komórka kostna

• Wydawca: Polskie Stowarzyszenie Biomateriałów
• Czasopismo: Inżynieria Biomateriałów
• Rocznik: 2006
• Tom: R. 9, nr 58-60
• Strony: 4--7
• Opis fizyczny: Bibliogr. 14 poz., rys., tab., wykr.

Twórcy

autor

Vagaska B.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Bacakova L.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Pamuła E.

• AGH-UST, Faculty of Materials Science and Ceramics, Department of BiomaterialsAl. Mickiewicza 30, 30-059 Kraków

autor

Lisa V.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Dobrzyński P.

• Centre for Polymer Chemistry, Polish Academy of Sciences, ul. Curie-Skłodowskiej 34/20, 41-819 Zabrze, Poland

Bibliografia

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• [2] Bacakova L., Jungova I., Slosarczyk A., Zima A., Paszkiewicz Z.: Inż Biomater – Eng Biomater, 7 [38-42]: 15-18, 2004.
• [3] Bryan DJ., Tang JB., Doherty SA., Hile DD., Trantolo DJ., Wise DL., Summerhayes IC.: J Neural Eng 1: 91-98, 2004.
• [4]Chung TW., Wang YZ., Huang YY., Pan CI., Wang SS.: Artif Organs 30:35-41 2006.
• [5] Dobrzynski P., Kasperczyk J.: J Pol Sci Part A, 44: 3184-3201, 2006.
• [6] Haas J., Ravi Kumar MN., Burchard G., Bakowsky U., Lehr CM.: AAPS PharmSciTech 6: E22-E30, 2005.
• [7] Han DW., Sub Lee M., Park BJ., Kim JK., Park JC.: Biotechnol Lett 27: 53-58, 2005.
• [8] Lysaght M., Hazlehurst A.: Tissue Eng 10: 309-320, 2004.
• [9] Pamuła E., Błażewicz M., Paluszkiewicz C., Dobrzyński P.: J Mol. Struct 596: 69-75, 2001.
• [10] Pamuła E., Buczyńska J.: Ceramika-Ceramics 91/1: 577-584, 2005.
• [11] Savaiano JK., Webster TJ.: Biomaterials 25: 1205-1213, 2004.
• [12] Venugopal J., Ma LL., Yong T., Ramakrishna S.: Cell Biol Int 29: 861-867, 2005.
• [13] Yoon JJ., Nam YS., Kim JH., Park TG: Biotechnol Bioeng 78: 1-10, 2002
• [14] Yoon JJ., Song SH., Lee DS., Park TG.: Biomaterials 25: 5613- 5620. 2004.