Adhesion and growth of vascular smooth muscle cells on protein assemblies for biomaterial coating

• Autorzy: Filova E. , Brynda E. , Houska M. , Riedel T. , Bacakova L.
• Języki publikacji: EN

Abstrakty

EN

We investigated adhesion and growth of vascular smooth muscle cells (VSMC) on proteins assemblies prepared by coating of thin fibrin net with ultrathin layer of collagen I, fibronectin or laminin. The assemblies were deposited on polystyrene (PS) non-modified with plasma discharge, i.e. a treatment commonly used for creation of surfaces suitable for cell cultivation. All samples with the fibrin assemblies improved initial attachment and spreading of VSMC as well as their subsequent growth in comparison with the pristine PS. The adhesion area of VSMC increased on fibrin coated with collagen I and fibronectin, while on laminin it remained similar as that on the pure fibrin. Thin 3D fibrin net markedly changed the morphology of VSMC from polygonal to star-like shape with protrusions. Cells all tested samples formed vinculin-containing focal adhesion plaques and α-actin cytoskeleton, an important marker of VSMC differentiation. The protein assemblies can be used for improvement of cell adhesion and growth on various artificial materials used in tissue engineering, e.g. for construction of bioartificial vascular prostheses.

Słowa kluczowe

EN

VSMC adhesion; ECM protein; focal adhesion; surface modification

• Wydawca: Polskie Stowarzyszenie Biomateriałów
• Czasopismo: Inżynieria Biomateriałów
• Rocznik: 2005
• Tom: R. 8, nr 47-53
• Strony: 9--12
• Opis fizyczny: Bibliogr. 7 poz., rys., wykr.

Twórcy

autor

Filova E.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 00 Prague 4-Krc, Czech Republic
• Centre of Cardiovascular Research, Videnska St. 1083, 142 00 Prague 4-Krc, Czech Republic

autor

Brynda E.

• Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic

autor

Houska M.

• Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic

autor

Riedel T.

• Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic

autor

Bacakova L.

• Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska St. 1083, 142 00 Prague 4-Krc, Czech Republic
• Centre of Cardiovascular Research, Videnska St. 1083, 142 00 Prague 4-Krc, Czech Republic

Bibliografia

• [1]. Bacáková L., Kunes. J.: Physiol. Res. 44: 127-130, 1995.
• [2]. Bacáková L, Walachová K, Svorcík V, Hnatowicz V., J. Biomater. Sci. Polym. Ed. 12: 817-834, 2001.
• [3]. Bacáková L, Filová E, Rypácek F, Svorcík V, Starý V.: Physiol. Res. 53: Suppl 1: S35-45, 2004.
• [4]. Benoit D.S., Anseth K.S.: Biomaterials. 26: 5209-5220, 2005. (RGD, PHSRN)
• [5]. Brodie J.C., Goldie E., Connel G., Merry J., Grant M.H.: J. Biomed. Mater. Res. A. 73: 409-421, 2005.
• [6]. Davis G. E., Bayless K.J., Mavila A: Anat. Rec. 268: 252-275, 2002.
• [7]. Heitz J., Svorcík V., Bacáková L. K. Rocková K., Ratajová E., Gumpenberger T., Bäuerle D., B. Dvoránková B., H. Kahr B., Graz I., Romanin C.: J. Biomed. Mater. Res. A. 67: 130-137, 2003.