Changes in activity of acid hydrolases in tissues of wild pheasants induced by heavy metals

• Autorzy: Zielińska S. , Pieniążek M. , Dżugan M.

Identyfikatory

DOI

10.2428/ecea.2015.22(1)09

Warianty tytułu

PL

Zmiany aktywności kwaśnych hydrolaz w tkankach dzikich bażantów wywołane przez metale ciężkie

• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determinate the activity of chosen acid hydrolases in tissues of pheasants (Phasianus colchicus L.) and first attempts to use these enzymes as biomarkers of the effect of birds exposure to heavy metals in the environment. The samples of liver, kidneys and testes were collected from wild pheasants shot in the Podkarapacie region in contaminated (n = 5) and clear (n = 5) areas. Tissues were examined for the cadmium and lead concentrations by AAS method, as well as the activity of five hydrolases. The major site of cadmium accumulation were kidneys, the levels of Cd found in liver and testes were by 5-fold and 30-fold lower (P < 0.05), respectively. Similarly, Pb concentrations observed in the liver and testes were lower than in kidney, by 1.5-fold and 6-fold, respectively. The presence of four glycosidases and arylsulphatase in all examined tissues was demonstrated and N-acetyl-β-D-glucosaminiadase (NAG) was identified as the most active enzyme in all studied tissues. Significant differences (P < 0.05) were observed only in the level of NAG activity in liver of pheasants inhabiting the contaminated area as compared to the birds from the clear region (control group). We conclude that the determination of NAG in birds organs can be used as biomarker of environmental exposure to cadmium.

PL

Celem badań było oznaczenie aktywności wybranych kwaśnych hydrolaz w tkankach bażantów (Phasianus colchicus L.) oraz zastosowanie po raz pierwszy tych enzymów jako biomarkerów narażenia środowiskowego ptaków na metale ciężkie. Próbki wątroby, nerek oraz jąder zostały pobrane z bażantów odstrzelonych na Podkarpaciu w rejonie zurbanizowanym (n = 5) i ekologicznym (n = 5). Analizy tkanek obejmowały oznaczenie zawartości metali ciężkich (Cd, Pb) metodą AAS oraz aktywności kwaśnych hydrolaz. Głównym narządem docelowym akumulacji metali ciężkich były nerki, zawartość kadmu w wątrobie oraz jądrach była niższa odpowiednio 5- i 30-krotnie. Podobną zależność zaobserwowano dla ołowiu, koncentracja tego metalu w jądrach i nerkach była niższa odpowiednio 1.5- i 6-krotnie. We wszystkich badanych tkankach stwierdzono obecność czterech glikozydaz i arylosulfatazy, przy czym najobfitszym źródłem enzymów były jądra, a najbardziej aktywnym enzymem we wszystkich tkankach była N-acetylo-β-D-glukozaminidaza (NAG). Wykazano istotne różnice (P < 0.05) w poziomie aktywności NAG w wątrobie bażantów bytujących na terenie zurbanizowanym w porównaniu do regionu ekologicznego. Aktywność NAG w tkankach ptaków może być przydatnym biomarkerem środowiskowego narażenia na kadm.

Słowa kluczowe

EN

pheasant; heavy metals; biomarker; acid hydrolases

PL

bażant; metale ciężkie; biomarker; kwaśne hydrolazy

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. A
• Rocznik: 2015
• Tom: Vol. 22, nr 1
• Strony: 93--101
• Opis fizyczny: Bibliogr. 28 poz., wykr., tab.

Twórcy

autor

Zielińska S.

• Department of Chemistry and Food Toxicology, Faculty of Biology and Agriculture, University of Rzeszów, ul. Ćwiklińskiej 2, 35–601 Rzeszów, Poland

autor

Pieniążek M.

• Faculty’s Laboratory, Faculty of Biology and Agriculture, University of Rzeszów, ul. Ćwiklińskiej 2, 35–601 Rzeszów, Poland.

autor

Dżugan M.

• Department of Chemistry and Food Toxicology, Faculty of Biology and Agriculture, University of Rzeszów, ul. Ćwiklińskiej 2, 35–601 Rzeszów, Poland

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