Encapsulated catalase from Serratia genus for H2O2 decomposition in food applications

• Autorzy: Czyzewska K. , Trusek A.

Identyfikatory

DOI

10.2478/pjct-2018-0052

• Języki publikacji: EN

Abstrakty

EN

The recombinant catalase isolated from a psychrotolerant microorganism belonging to Serratia genus exhibits a high activity in a wide range of pH. Due to a great catalytic potential in operational conditions, it can be used in various industrial applications whereby it acts as a hydrogen peroxide scavenger. To reduce the cost of biocatalyst the enzyme encapsulation into a hydrogel structure was proposed. The obtained results showed a high activity of encapsulated catalase in acidic conditions (pH in range 4.4 - 6.6) and at low temperatures (6-15°C). Moreover, immobilized catalase exhibited a high stability in natural media, especially in milk. Its activity during peroxide decomposition in milk, the possibility of re-using, as well as the fixed bed reactor performance confirmed wide application possibilities. High values of enzyme and substrate concentrations led to the beads burst due to rapid oxygen diffusion from the capsules, thus they are limited.

Słowa kluczowe

EN

catalase; hydrogen peroxide; enzyme encapsulation; cold sterilization; Serratia genus

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2018
• Tom: Vol. 20, nr 4
• Strony: 39--43
• Opis fizyczny: Bibliogr. 27 poz., rys., tab.

Twórcy

autor

Czyzewska K.

• Wroclaw University of Science and Technology, Department of Bioprocess and Biomedical Engineering, Norwida 4/6, 50-373 Wroclaw, Poland

autor

Trusek A.

• Wroclaw University of Science and Technology, Department of Bioprocess and Biomedical Engineering, Norwida 4/6, 50-373 Wroclaw, Poland

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).