Development and validation of a rapid HPLC method for multiresidue determination of erythromycin, clarithromycin, and azithromycin in aquaculture fish muscles

• Autorzy: Habibi B. , Ghorbel-Abid I. , Lahsini R. , Ben Hassen D. Chehimi , Trabelsi-Ayadi M.

Identyfikatory

DOI

10.1556/1326.2017.00376

• Języki publikacji: EN

Abstrakty

EN

An accurate, sensitive, and reproducible high-performance liquid chromatographic method with diode array detection has been developed for simultaneous determination of erythromycin, clarithromycin, and azithromycin residues in fish muscles. Analysis was carried out using a Shodex Asahipak high-performance liquid chromatography (HPLC) column, monitoring was at 210 nm and a mobile phase consisting of a mixture of acetonitrile and phosphate buffer (pH 11 ± 0.05) in the ratio of 60:40 (v/v). Solid-phase extraction method was used in samples extraction and purification. Recoveries were in the range 72.0–92.2% with relative standard deviation (RSD) from 2.3% to 8.3%. This method was validated for fish muscles in aquaculture following the commission decision 2002/657/EC criteria. It is demonstrated that the new method is robust for detection and quantification of the three macrolides residues. Decision limit (CCα) was from 214 to 228 μg/kg and capacity of detection (CCβ) was from 228 to 256 μg/kg.

Słowa kluczowe

EN

Macrolides; residues; fish; HPLC-DAD; SPE; validation

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 2
• Strony: 109--112
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Habibi B.

• Laboratory of Materials Processing and Analysis (LMTA), National Institute for Research, Physical and Chemical Analysis (INRAP), Biotech Pole Sidi-Thabet, 2032 Ariana, Tunisia
• Laboratory of Research and Development, SIPHAT, BenArous 2036, Tunisia

autor

Ghorbel-Abid I.

• Laboratory of Materials Processing and Analysis (LMTA), National Institute for Research, Physical and Chemical Analysis (INRAP), Biotech Pole Sidi-Thabet, 2032 Ariana, Tunisia
• Faculty of Sciences of Bizerte, Laboratory of Chemistry, Applications to Natural Resources Substances and Environment, LACReSNE, University of Carthage, 7021 Zarzouna, Bizerte, Tunisia

autor

Lahsini R.

• Laboratory of Research and Development, SIPHAT, BenArous 2036, Tunisia

autor

Ben Hassen D. Chehimi

• Laboratory of Materials Processing and Analysis (LMTA), National Institute for Research, Physical and Chemical Analysis (INRAP), Biotech Pole Sidi-Thabet, 2032 Ariana, Tunisia

autor

Trabelsi-Ayadi M.

• Laboratory of Materials Processing and Analysis (LMTA), National Institute for Research, Physical and Chemical Analysis (INRAP), Biotech Pole Sidi-Thabet, 2032 Ariana, Tunisia

Bibliografia

• [1] Guardabassi, L.; Jensen Lars, B.; Kruse, H. Guide to Antimicrobial Use in Animals April 2008, Wiley-Blackwell.
• [2] Cristofani, E.; Antonini, C.; Tovo, G.; Fioroni, L.; Piersanti, A.; Galarini, R. Analytica. Chimica. Acta 2009, 637, no. 1–2, pp. 40–46.
• [3] Gonźalez de la Huebra, M. J.; Vincent, U. J. Pharm. Biomed. Anal. 2005, 39, pp. 376–398.
• [4] Sismotto, M.; Jonas Paschoal, A. R.; Teles, Juliana A.; Estaiano, Renata A. R.; Felix Reyes, G. R. J. Food Comp. Anal. 2014, 34 (2), pp. 153–162.
• [5] Suárez, A.; Ellis, R. FAO/WHO Expert Committee 66th Meeting 29, 2006.
• [6] Sekkin, S.; Cavit, A. Antibacterial Drugs in Fish Farms: Application and Its Effects; Adnan Menderes University: Turkey, page 233.
• [7] Guidelines for Prevention and Management of Common Opportunistic Infections/Malignancies among HIV-Infected Adult and Adolescent Ministry of Health & Family Welfare Government of India May 2007.
• [8] Shalf, H. J. Pharm. Res. 4, pp. 278–283.
• [9] Horie, M.; Takegami, H.; Toya, K.; Nakazawa, H. Analytical Chemica. Acta 2003, 492, 187–197.
• [10] Simon, B.; Hancu, G.; Braz, Á. G. J. Pharm. Sci. July/Sept. 2014, 50, São Paulo, 521–527.
• [11] Wang, J.; Leung, D.; Lenz, S. P. J. Agric. Food Chem. 2006, 54, pp. 2873–2880.
• [12] Lahane, S. B.; Deokate, U. A.; Ahire, S. K. Int. J. Pharm. Sci. Rev. Res. 2014, 26, No. 44, pp. 256–261.
• [13] Gaugain, M. J.; Anger, B. J. AOAC Int. 1999, 82, pp. 1046.
• [14] Shankar, B. P.; Manjunatha Prabhu, B. H.; Chandan, S.; Ranjith, ; Shivakumar, V. Veterinary World Vol. 2010, 3, pp. 241–246.
• [15] Yu, H.; Tao, Y.; Chen, D.; Wang, Y.; Yuan, Z. Food Chem. 2011, 124, pp. 1131–1138.
• [16] Commission Decision of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (2002/657/EC). Official Journal of the European Communication L221, 2002, 8.
• [17] Lahsini, R.; Monser, L. Acta. Chromatogr. 2015, 27, pp. 449–460.
• [18] Lahsini, R.; Monser, L. Pharm. Chem. J. 2012, 46, pp. 127–131.
• [19] Carretero, V.; Balsco, C.; Pico, Y. , J. Chromatogr. A 2008, 1209, pp. 162–173.
• [20] Taoa, Y.; Yua, G.; Chena, D.; Pana, Y.; Liua, Z.; Wei, H.; Penga, D.; Huang, L.; Wanga, Y.; Yuana, Z. J. Chromatogr. B 2012, 897, pp. 64–71.
• [21] Cinquina, A. L.; Longo, F.; Anastasi, G.; Giannetti, L.; Cozzani, R. J. Chromatogr. A 2003, 987, pp. 227–233, 2003.
• [22] Zhou, J.; Xue, X.; Li, Y.; Zhang, J.; Chen, F.; Wu, L. Food Chem. 2009, 115, pp. 1074–1080.
• [23] Shalaby, A. R.; Salama Nadia, A.; Abou-Raya, S. H.; Wafaa Emama, H.; Mehaya, F. M. Food Chem. 2011, 124, pp. 1660–1666.
• [24] Hellal, N.; Monser, L. J. Sep. Science 2008, 31, pp. 276–282.

Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).