Effect of Growth Medium Composition on the Physicochemical Surface Properties of Pseudomonas savastanoi, the Agent of Olive Tuberculosis

• Autorzy: Mitro Soukaina , El Othmany Rabha , Hakim Taoufik , Zanane Chorouk , Lekchiri Souad , Mazigh Doha , El Louali Mostafa , Latrache Hassan , Zahir Hafida

Identyfikatory

DOI

10.12912/27197050/189240

• Języki publikacji: EN

Abstrakty

EN

Research into the formation of Pseudomonas savastanoi biofilms on olive trees is essential to prevent infections that induce tumour formation and damage tree health. To prevent the development of P. savastanoi biofilms, it is crucial to comprehend the factors influencing its adhesive behaviour. This research analysed the physicochemical properties of P. savastanoi in two types of media. P. savastanoi was cultivated in two media: nutrient agar (NA) and King B (KB), in solid and liquid forms. Wettability (θw), electron acceptor (γ+) electron donor (γ- ) properties, and surface free energy (ΔGiwi) are evaluated by contact angle measurements. The obtained results indicated that in solid media (NA and KB), P. savastanoi exhibited hydrophobic surface (𝜃_W^NA = 82.23°; 𝜃_W^KB= 94.9°), with strong electron donor (γ_NA⁻ = 43.54 mJ.m^-2; γ_KB⁻ = 58.34 mJ·m^-2) and mild electron acceptor (γ_NA⁺ = 0.32 mJ.m^-2; γ_KB⁺ = 0.52 mJ·m^-2) properties, along with negative surface free energy (ΔG_iwi^NA-76.13 mJ·m^-2; ΔG_iwi^KB= -65.33 mJ·m^-2. Conversely, in liquid media (NB and KB), the surface of P. savastanoi was generally hydrophilic (𝜃_W^NB= 55.43°; 𝜃_W^KB = 64.43°), with strong electron donor (γ_NB⁻ = 29.23 mJ.m^-2; γ_KB⁻ = 41.38 mJ.m^-2) and a surface free energy that registers as positive. Modification of the growth medium composition led to minor variations in P. savastanoi hydrophobicity and surface free energy. By understanding the factors involved in this adhesive behaviour, ecological methods to protect crops and contribute to more efficient environmental management and conservation of natural resources can be developed.

Słowa kluczowe

EN

electron donor; electron acceptor; hydrophobicity; Pseudomonas savastanoi; olive tree; contact angle measurements

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2024
• Tom: Vol. 25, iss. 8
• Strony: 72--78
• Opis fizyczny: Bibliogr. 35 poz., tab.

Twórcy

autor

Mitro Soukaina

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0003-0049-5165

autor

El Othmany Rabha

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

autor

Hakim Taoufik

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0002-2229-9205

autor

Zanane Chorouk

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0002-6334-5386

autor

Lekchiri Souad

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0002-9517-2512

autor

Mazigh Doha

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0001-6285-0172

autor

El Louali Mostafa

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0002-1337-885X

autor

Latrache Hassan

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0003-2297-9320

autor

Zahir Hafida

• Laboratory of Industrial and Surface Engineering, Bioprocesses and Biointerfaces Team, Faculty of Science and Techniques, Sultan Moulay Slimane University, PO BOX 523 Beni Mellal, Morocco

• https://orcid.org/0000-0001-8663-1775

Bibliografia

• 1. Ater, M., Barbara, H., Kassout, J. 2016. Importance des variétés locales, de l’oléastre et des pratiques traditionnelles de l’oléiculture dans la région de Chefchaouen (Nord du Maroc). L’Oléiculture au Maroc de la préhistoire à nos jours: pratiques, diversité, adaptation, usages, commerce et politiques, 109-121.
• 2. Briandet, R., Meylheuc, T., Maher, C., Bellon-Fontaine, M.N.L. 1999. Listeria monocytogenes Scott A: cell surface charge, hydrophobicity, and electron donor and acceptor characteristics under different environmental growth conditions. Applied and environmental microbiology, 65(12), 5328–5333. https://doi.org/10.1128/AEM.65.12.5328-5333.1999
• 3. Busscher, H.J., Weerkamp, A.H., van der Mei, H.C., Van Pelt, A., de Jong, H.P., Arends, J. 1984. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Applied and environmental microbiology, 48(5), 980–983. https://doi.org/10.1128/aem.48.5.980-983.1984
• 4. Chliyeh, M., Touati, J., Selmaoui, K., Touhami, A.O., Filali-Maltouf, A., El Modafar, C. 2014. Bibliographic inventory of the olive tree (Olea europaea L.) fungal diseases in the world. Indian Journal of Pure & Applied Biosciences, 2, 46–79.
• 5. Dang, H., Lovell, C.R. 2016. Microbial surface colonization and biofilm development in marine environments. Microbiology and molecular biology reviews, 80(1), 91–138. https://doi.org/10.1128/mmbr.00037-15
• 6. Debbab, M., El-Hajjaji, S., Aly, A.H., Dahchour, A., El Azzouzi, M., Zrineh, A. 2014. Cypermethrin residues in fresh vegetables: Detection by HPLC and LC-ESIMS and their effect on antioxidant activity. Journal of Materials and Environmental Science, 5(S1), 2257–2266.
• 7. Debbabi, O.S., Amar, F.B., Rahmani, S.M., Taranto, F., Montemurro, C., Miazzi, M.M. 2022. The status of genetic resources and olive breeding in Tunisia. Plants, 11(13), 1759. https://doi.org/10.3390/plants11131759
• 8. Eisen, A., Reid, G. 1989. Effect of culture media on Lactobacillus hydrophobicity and electrophoretic mobility. Microbial ecology, 17, 17–25.
• 9. El Bakkali, A., Essalouh, L., Tollon, C., Rivallan, R., Mournet, P., Moukhli, A., Zaher, H., Mekkaoui, A., Hadidou, A., Sikaoui, L., Khadari, B. 2019. Characterization of worldwide olive germplasm banks of Marrakech (Morocco) and Córdoba (Spain): Towards management and use of olive germplasm in breeding programs. PLoS One, 14(10), e0223716. https://doi.org/10.1371/journal.pone.0223716
• 10. El Mouhtadi, I., Agouzzal, M., Guy, F. 2014. L’olivier au Maroc. Oilseeds and fats, Crops and Lipids, 21(2), D203. http://dx.doi.org/10.1051/ocl/2013053
• 11. El Qarnifa, S., El Antari, A., Hafidi, A. 2019. Effect of maturity and environmental conditions on chemical composition of olive oils of introduced cultivars in Morocco. Journal of Food Quality, 2019, 1–14. https://doi.org/10.1155/2019/1854539
• 12. Gallardo-Moreno, A., Gonzalez-Martin, M., Bruque, J., Perez-Giraldo, C., Sanchez-Silos, R., Gomez-Garcia, A. 2003. Influence of the growth medium, suspending liquid and measurement temperature on the physico-chemical surface properties of two Enterococci strains. Journal of adhesion science and technology, 17(14), 1877–1887. https://doi.org/10.1163/156856103770572034
• 13. Gallardo-Moreno, A.M., González-Martín, M.L., Bruque, J.M., Pérez-Giraldo, C. 2004. Changes on the physico-chemical surface properties and adhesion behaviour of Enterococcus faecalis by the addition of serum or urine to the growth medium. Physical Chemistry Chemical Physics, 6(7), 1512–1517. https://doi.org/10.1039/B314609J
• 14. Hamadi, F., Latrache, H., El Ghmari, A., Ellouali, M., Mabrrouki, M., Kouider, N. 2004. Effect of pH and ionic strength on hydrophobicity and electron donor and acceptor characteristics of Escherichia coli and Staphylococcus aureus. Annals of Microbiology, 54, 213–226.
• 15. Hamadi, F., Latrache, H., Elghmari, A., Zahir, H., Mabrrouki, M., Elbouadili, A.E. 2005. Determination of Escherichia coli negative charge concentration from XPS data and its variation with pH. Journal of surface analysis, 12(3), 293.
• 16. Hori, K., Matsumoto, S. 2010. Bacterial adhesion: From mechanism to control. Biochemical Engineering Journal, 48(3), 424–434. https://doi.org/10.1016/j.bej.2009.11.014
• 17. Kankaanpaa, P., Yang, B., Kallio, H., Isolauri, E., Salminen, S. 2004. Effects of polyunsaturated fatty acids in growth medium on lipid composition and on physicochemical surface properties of Lactobacilli. Applied and environmental microbiology, 70(1), 129–136. https://doi.org/10.1128/AEM.70.1.129-136.2004
• 18. Kawsar, S.M., Kabir, A.K., Manik, M.M., Hossain, M.K., Anwar, M.N. 2012. Antibacterial and mycelial growth inhibition of some acylated derivatives of D-glucopyranoside.
• 19. Kebede, G., Tafese, T., Abda, E.M., Kamaraj, M., Assefa, F. 2021. Factors influencing the bacterial bioremediation of hydrocarbon contaminants in the soil: mechanisms and impacts. Journal of Chemistry, 2021, 1–17. https://doi.org/10.1155/2021/9823362
• 20. Lahlali, R., Ezrari, S., Radouane, N., Kenfaoui, J., Esmaeel, Q., El Hamss, H., Belabess, Z., Barka, E.A. 2022. Biological control of plant pathogens: A global perspective. Microorganisms, 10(3), 596. https://doi.org/10.3390/microorganisms10030596
• 21. Latrache, H., Bourlioux, P., Karroua, M., Zahir, H., Hakkou, A. 2000. Effects of subinhibitory concentrations of nitroxoline on the surface properties of Escherichia coli. Folia microbiologica, 45, 485– 490. https://doi.org/10.1007/BF02818714
• 22. Latrache, H., El, G., Karroua, M., Hakkou, A., Ait, M.H., El, B., et al. 2002. Relations between hydrophobicity tested by three methods and surface chemical composition of Escherichia coli. The new microbiologica, 25(1), 75–82.
• 23. Latrache, H., Mozes, N., Pelletier, C., Bourlioux, P. 1994. Chemical and physicochemical properties of Escherichia coli: variations among three strains and influence of culture conditions. Colloids and surfaces B: Biointerfaces, 2(1–3), 47–56. https://doi.org/10.1016/0927-7765(94)80017-0
• 24. Mathez, A., Loftus, A. 2023. Endless modernisation: Power and knowledge in the Green Morocco Plan. Environment and Planning E: Nature and Space, 6(1), 87–112. https://doi.org/10.1177/25148486221101541
• 25. Mceldowney, S., Fletcher, M. 1986. Effect of growth conditions and surface characteristics of aquatic bacteria on their attachment to solid surfaces. Microbiology, 132(2), 513–523. https://doi.org/10.1099/00221287-132-2-513
• 26. Meftah, H., Boughdad, A., Bouchelta, A. 2014. Infestation et cycle biologique d’Euphyllura olivina Costa (Homoptera, Psyllidae) au centre du Maroc. ScienceLib éditions Mersenne, 6, 1–25.
• 27. Ngwai, Y., Sabiya, G. 2007. Cultivation in different growth media affects the expression of the cell surface hydrophobicity of bacteria. Cameroon Journal of Experimental Biology, 3(1), 26–29.
• 28. Rongai, D., Milano, F., Sciò, E. 2012. Inhibitory effect of plant extracts on conidial germination of the phytopathogenic fungus Fusarium oxysporum. doi: http://www.scirp.org/journal/PaperInformation.aspx?PaperID=25782
• 29. Ruano-Rosa, D., Valverde-Corredor, A., Gómez-Lama Cabanás, C., Sesmero, R. and Mercado-Blanco, J. 2017. What lies beneath: Root-associated bacteria to improve the growth and health of olive trees. In Soil Biological Communities and Ecosystem Resilience (pp. 107–122). Springer International Publishing. https://doi.org/10.1007/978-3-319-63336-7_7
• 30. Sepehrnia, N., Gorakifard, M., Hallett, P.D., Hajabbasi, M.A., Shokri, N., Coyne, M. 2023. Contrasting transport and fate of hydrophilic and hydrophobic bacteria in wettable and water-repellent porous media: Straining or attachment? Colloids and Surfaces B: Biointerfaces, 228, 113433. https://doi.org/10.1016/j.colsurfb.2023.113433
• 31. Van Oss, C. 1990. Mechanisms of and conditions for repulsive van der uaals and repulsive hydrogen bonding interactions. Journal of dispersion science and technology, 11(5), 491–502. https://doi.org/10.1080/01932699008943273
• 32. Van Oss, C. 1993. Acid-base interfacial interactions in aqueous media. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 78, 1–49. https://doi.org/10.1016/0927-7757(93)80308-2
• 33. Van Oss, C.J., Chaudhury, M.K., Good, R.J. 1988. Interfacial Lifshitz-van der Waals and polar interactions in macroscopic systems. Chemical reviews, 88(6), 927–941. https://doi.org/10.1021/cr00088a006
• 34. Zahir, I. 2016. Effect of Moroccan plants against phytopathogenic microorganisms: a review. British Biotechnology Journal, 10(1), 1–36. https://doi.org/10.9734/BBJ/2016/21430
• 35. Zouiten, N., El Hadrami, I. 2001. La psylle de l’olivier: état des connaissances et perspectives de lutte. Cahiers Agricultures, 10(4), 225–232.