An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue

• Autorzy: Rzepecka P. , Duda P. , Giebułtowicz J. , Sochacka M. , Friedman A. , Gałązka-Friedman J.

Identyfikatory

DOI

10.1515/nuka-2017-0024

• Konferencja: All-Polish Seminar on Mössbauer Spectroscopy OSSM 2016 (11th ; 19-22 June 2016 ; Radom-Turno, Poland)
• Języki publikacji: EN

Abstrakty

EN

Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investigations. Biological samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude possible artefacts from the background spectrum related to iron present in the counter and cryostat windows. The aim of this study was to compare two methods of determination of the amounts of iron in investigated sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the other, in which the obtained non-elaborated spectrum was fitted with two doublets – a doublet for the measured sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron (400, 800 and 1600 µg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed. Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99. Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the background spectrum had a significantly lower error of the final result.

Słowa kluczowe

EN

Mössbauer spectroscopy; background spectrum; iron concentration; brain tissue; calibration curve

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2017
• Tom: Vol. 62, No. 2
• Strony: 159--163
• Opis fizyczny: Bibliogr. 8 poz., rys.

Twórcy

autor

Rzepecka P.

• Faculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662 Warsaw, Poland

autor

Duda P.

• Faculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662 Warsaw, Poland

autor

Giebułtowicz J.

• Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland

autor

Sochacka M.

• Faculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662 Warsaw, Poland

autor

Friedman A.

• Department of Neurology, Faculty of Health Science, Medical University of Warsaw, Warsaw, Poland

autor

Gałązka-Friedman J.

• Faculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662 Warsaw, Poland

Bibliografia

• 1. Herr, W., & Skerra, B. (1969). Mössbauer spectroscopy applied to the classification of stone meteorites. In: P. M. Millman (Ed.), Meteorite research (Vol. 12, pp. 106–122). Astrophysics and Space Series. Science Library. Dordrecht: D. Reidel Pub. Co.
• 2. Morris, R. V., Klingelhöfer, G., Korotev, L. R., & Shelfer, T. D. (1998). Mössbauer mineralogy on the Moon: The lunar regolith. Hyperfi ne Interact., 117, 405–432.
• 3. Bauminger, E. R., Barcikowska, M., Friedman, A., Hechel, D., & Nowik, I. (1994). Does iron play a role in Parkinson’s disease? Hyperfi ne Interact., 91, 853–856.
• 4. Dubiel, S. M., Zabłotna-Rypień, B., Mackey, J. B., & Williams, J. M. (1999). Magnetic properties of human liver and brain ferritin. Eur. Biophys. J., 28, 263–267.
• 5. Gerlach, M., Trautwein, A. X., Zecca, L., Youdim, M. B. H., & Riederer, P. (1995). Mössbauer spectroscopic studies of purified human neuromelanin isolated from the substantia nigra. J. Neurochem., 65, 923–926.
• 6. Galazka-Friedman, J., Bauminger, E. R., Koziorowski, D., & Friedman, A. (2004). Mössbauer spectroscopy and ELISA studies reveal differences between Parkinson’s disease and control substantia nigra. Biochim. Biophys. Acta, 1688, 130–136.
• 7. Zecca, L., Gallorini, M., Schunemann, V., Trautwein, A. X., Gerlach, M., Riederer, P., Vezzoni, P., & Tampellini, D. (2001). Iron, neuromelanin and ferritin content in the substantia nigra of normal subjects at different ages: consequences for iron storage and neurodegenerative processes. J. Neurochem., 76(6), 1766–1773.
• 8. Miglierini, M., Rocca, R., Kopani, M., & Lancok, A. (2014). Mössbauer and SQUID characterization of iron in human tissue: case of globus pallidus. Acta Phys. Pol. A, 126, 240–241.

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).