Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway

• Autorzy: Atamanalp Muhammed , Kırıcı Muammer , Köktürk Mine , Kirici Mahinur , Alwazeer Duried , Kocaman Esat Mahmut , Ucar Erzu , Parlak Veysel , Özcan Sinan , Alak Gonca

Identyfikatory

DOI

10.26881/oahs-2023.2.05

• Języki publikacji: EN

Abstrakty

EN

Although the number of studies documenting the presence of Microplastics (MP) in fish is increasing, research studies focused on its detoxification are very limited. In this study, rainbow trout ( Oncorhyncus mykiss) were randomly divided into two groups after being fed with MPs (15% polypropylene [PP] +15% polyethylene [PE]) for 2 months. MP excretion without any application (PP+PE) in group I fish, and excretion of MPs with hydrogen-rich water (HRW) application (PP+PE+HRW) in group 2 were investigated under semi-static conditions for 21 days. This effectwas also compared by using positive and negative control groups (Control[no treatment, free PP, PE or/and HRW] and only HRW group). In thisdirection, the following were determined: PP+PE chronic toxicity in aquatic organisms, the toxicity mechanism and the effect of HRW as a possibletreatment method in blood tissue; with hematological indices ([RBC count [RBC], leukocyte count [WBC], hemoglobin value [Hb], hematocrit ratio [Hct], platelet count [PLT], hemoglobin count per erythrocyte [MCHC],mean hemoglobin amount per erythrocyte [MCH] and mean erythrocyte volume [MCV]) in other tissues (liver, gill and brain tissue) oxidative stress response (catalase [CAT]), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), glutathione (GSH), reactive oxygen products (ROS), malondialdehyde ([MDA] levels), DNA damage (8-OHdG: 8-Hydroxy-2-Deoxyguanosine), and the apoptosis (caspase 3) levels were investigated. In addition, acetylcholinesterase enzyme (AChE) activity, which is important in neurotoxicity pathways in the brain, wasdetermined. The presence of plastics (PP/PE) in target tissues (muscle, liver,gill and gastrointestinal tract) was also obtained.The results showed that PP+PE caused toxicity in all three tissues. MPs showed an inhibiting effect on antioxidant enzyme activities and an inductive effect on MDA, ROS, 8-OHdG, and caspase 3 levels. HRW showed a mitigating effect on MP-mediated toxicity in O. mykiss brain, blood, gill, and liver by controlling the ROS/ GSH/MDA pathway. HRW can be suggested as a cost-effective and eco-friendly curative for the protection of fish from the oxidative damages produced by the ingestion of microplastics.

Słowa kluczowe

EN

oxy-hydrogen gas; hydrogen-richwater; molecular hydrogen; MPs/NPs; bloodindices; rainbow trout; antioxidant status; fish

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2023
• Tom: Vol. 52, No. 2
• Strony: 206--220
• Opis fizyczny: Bibliogr. 56 poz., fot., tab., wykr.

Twórcy

autor

Atamanalp Muhammed

• Department of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030 Erzurum, Türkiye

autor

Kırıcı Muammer

• Department of Veterinary Health, Food Agriculture and Livestock Vocational School, Bingöl University, Bingöl 12000, Türkiye

autor

Köktürk Mine

• Department of Organic Agriculture Management, Faculty of Applied Sciences, Igdir University, TR-76000 Igdir, Türkiye

autor

Kirici Mahinur

• Department of Chemistry, Faculty of Arts and Science, Bingöl University, Bingöl 12000, Türkiye

autor

Alwazeer Duried

• Research Center for Redox Applications in Foods (RCRAF), Igdir University, 76000, Igdir, Turkey. Innovative Food Technologies Development, Application, and Research Center, Igdir University, 76000 Igdir, Türkiye

autor

Kocaman Esat Mahmut

• Department of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030 Erzurum, Türkiye

autor

Ucar Erzu

• Department of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030 Erzurum, Türkiye

autor

Parlak Veysel

• Department of Basic Sciences, Faculty of Fisheries, Ataturk University, Erzurum 25240, Türkiy

autor

Özcan Sinan

• Department of Aquaculture, Faculty of Fisheries, Atatürk University, TR-25030 Erzurum, Türkiye

autor

Alak Gonca

• Department of Seafood Processing Technology, Faculty of Fisheries, Ataturk University, Erzurum, Türkiye

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).