Composite scaffolds enriched with calcium carbonate microparticles loaded with epigallocatechin gallate for bone tissue regeneration

• Autorzy: Pietryga Krzysztof , Panaite Anca-Alexandra , Pamuła Elżbieta

Identyfikatory

DOI

10.34821/eng.biomat.166.2022.12-21

• Języki publikacji: EN

Abstrakty

EN

There is a need to develop advanced multifunctional scaffolds for the treatment of bone tissue lesions, which apart from providing support for infiltrating cells could assure the delivery of drugs or biologically active molecules enhancing bone formation. We developed composite scaffolds for bone tissue engineering based on gellan gum (GG) and gelatin (Gel) hydrogel enriched with epigallocatechin gallate (EGCG) loaded CaCO3 microparticles and subjected to enzymatic mineralization with calcium phosphate (CaP). The method of manufacturing CaCO3 microparticles was optimized. The EGCG-loaded microparticles were smaller than those unloaded, and the release of EGCG was prolonged for up to 14 days, as shown by the Folin-Ciocalteu test. The particles reduced the viability of the MG-63 cells as compared to the control. However, when they were loaded with EGCG, their cytotoxicity was reduced. The particles were suspended in a GG/Gel hydrogel containing alkaline phosphatase (ALP), soaked in calcium glycerophosphate (CaGP) solution to create CaP deposits, and submitted to freeze-drying, in order to produce a porous scaffold. The microstructure of the scaffolds was characterized by optical and scanning electron microscopy and showed that the size of the pores corresponds to that of the spongy bone. In vitro tests with MG-63 cells confirmed that mineralized scaffolds support cell adhesion and growth to a higher extent than nonmineralized ones.

Słowa kluczowe

EN

gellan gum; gelatin; hydrogel; calcium carbonate microparticles; enzymatic mineralization; epigallocatechin gallate (EGCG)

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2022
• Tom: vol. 25, no. 166
• Strony: 12--21
• Opis fizyczny: Bibliogr. 13 poz., rys., wykr.

Twórcy

autor

Pietryga Krzysztof

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, Al. Mickiewicza 30, 30-059 Kraków, Poland
• Silesian Park of Medical Technology Kardio-Med Silesia, ul. Marii Skłodowskiej-Curie 10C, 41-800 Zabrze, Poland

• https://orcid.org/0000-0002-6343-8849

autor

Panaite Anca-Alexandra

• University Politechnica of Bucharest, Faculty of Medical Engineering, Splaiul Independentei 313, 060042 Bucharest, Romania

• https://orcid.org/0009-0007-4073-370X

autor

Pamuła Elżbieta

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials and Composites, Al. Mickiewicza 30, 30-059 Kraków, Poland

• https://orcid.org/0000-0002-0464-6189

Bibliografia

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• [3] Jiang S., Wang M., He J.: A review of biomimetic scaffolds for bone regeneration: Toward a cell-free strategy. Bioeng Transl Med 6 (2021) https://doi.org/10.1002/btm2.10206.
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• [10] Sovova S., Abalymov A., Pekar M., Skirtach A.G., Parakhonskiy B.: Calcium carbonate particles: synthesis, temperature and time influence on the size, shape, phase, and their impact on cell hydroxyapatite formation. J Mater Chem B 9 (2021) 8308–8320. https://doi.org/10.1039/ D1TB01072G.
• [11] Honda Y., Takeda Y., Li P., Huang A., Sasayama S., Hara E., et al.: Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering. Molecules 23:876 (2018) https://doi.org/10.3390/molecules23040876.
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• [13] Pietryga K., Costa J., Pereira P., Douglas T.E.L.: Promotion of bone cells growth on gellan gum hydrogels by enzymatic mineralization. Eng Biomat 125 (2014) 6–12.

Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu „Społeczna odpowiedzialność nauki” - moduł: Popularyzacja nauki i promocja sportu (2022-2023)