Bacterial Community of Neutral Mine Drainage of Elizabeth’s Shaft (Slovinky, Slovakia)

• Autorzy: Kiskova Jana , Perhacova Zuzana , Vlcko Ladislav , Sedlakova-Kadukova Jana , Kvasnova Simona , Pristas Peter

Identyfikatory

DOI

10.29227/IM-2019-01-16

Warianty tytułu

PL

Bakteryjna wspólnota neutralnej kopalni Drenaż szybu Elizabeth's (Slovinky, Słowacja)

• Języki publikacji: EN

Abstrakty

EN

Neutral mine drainage is the less frequent subject of the interest than acid mine drainage but it can have adverse environmental effects caused mainly by precipitation of dissolved Fe. The aim of the study was to characterize the composition of bacterial population in environment with high concentration of iron and sulfur compounds represented by neutral mine drainage water of Elizabeth’s shaft, Slovinky (Slovakia). The pH value of drainage water decreased from 7.1 to 6.5 during the years 2008–2014. Direct microscopic observations, cultivation methods, and 454 pyrosequencing of the 16S rRNA gene amplicons were used to examine the bacterial population. Microscopic observations identified iron–oxidizing Proteobacteria of the genera Gallionella and Leptothrix which occurrence was not changed during the years 2008–2014. Using 454 pyrosequencing, there were identified members of 204 bacterial genera that belonged to 25 phyla. Proteobacteria (69.55%), followed by Chloroflexi (10.31%) and Actinobacteria (4.24%) dominated the bacterial community. Genera Azotobacter (24.52%) and Pseudomonas (14.15%), followed by iron–oxidizing Proteobacteria Dechloromonas (11%) and Methyloversatilis (8.53%) were most abundant within bacterial community. Typical sulfur bacteria were detected with lower frequency, e.g., Desulfobacteraceae (0.25%), Desulfovibrionaceae (0.16%), or Desulfobulbaceae (0.11%). Our data indicate that the composition of bacterial community of the Elizabeth’s shaft drainage water reflects observed neutral pH, high level of iron and sulfur ions in this aquatic habitat.

PL

Odciek kopalniany o odczynie obojętnym jest rzadszym przedmiotem zainteresowania niż odciek kopalniany kwaśny, ale może mieć niekorzystne skutki środowiskowe spowodowane głównie przez wytrącanie rozpuszczonego Fe. Celem artykułu jest scharakteryzowanie składu bakterii w środowisku o wysokim stężeniu związków żelaza i siarki reprezentowanych przez obojętne wody drenażowe kopalni szybu Elizabeth, Slovinky (Słowacja). Wartość pH wody drenażowej spadła z 7,1 do 6,5 w latach 2008–2014. Bezpośrednie obserwacje mikroskopowe, metody hodowli i pirosekwencjonowanie amplikonów genu 16S rRNA wykorzystano do zbadania populacji bakterii. Obserwacje mikroskopowe zidentyfikowały proteobakterie utleniające żelazo z rodzajów Gallionella i Leptothrix, których występowanie nie uległo zmianie w latach 2008–2014. Przy użyciu pirosekwencjonowania 454 zidentyfikowano 204 rodzajów bakterii należących do 25 typów. Proteobakterie (69,55%), a następnie Chloroflexi (10,31%) i aktynobakte rie (4,24%) zdominowały społeczność bakteryjną. Rodzaje Azotobacter (24,52%) i Pseudomonas (14,15%), a następnie proeto-bakterie żelazo utleniające Dechloromonas (11%) i Methyloversatilis (8,53%) były najbardziej rozpowszechnione w społeczności bakteryjnej. Typowe bakterie siarkowe wykryto z mniejszą częstotliwością, np. Desulfobacteraceae (0,25%), Desulfovibrionaceae (0,16%) lub Desulfobulbaceae (0,11%). Uzyskane dane wskazują, że skład flory bakteryjnej wody drenażowej szybu Elżbieta odzwierciedla obserwowane neutralne pH, wysoki poziom zawartości jonów żelaza i siarki w środowisku wodnym.

Słowa kluczowe

EN

bacterial community; neutral mine drainage; high-throughput sequencing

PL

flora bakteryjna; neutralny drenaż kopalniany; sekwencjonowanie

• Wydawca: Polskie Towarzystwo Przeróbki Kopalin
• Czasopismo: Inżynieria Mineralna
• Rocznik: 2019
• Tom: R. 21, nr 1
• Strony: 89--94
• Opis fizyczny: Bibliogr. 27 poz., tab., wykr.

Twórcy

autor

Kiskova Jana

• Pavol Jozef Šafárik University in Košice, Faculty of Science, Institute of Biology and Ecology, Šrobárova 2, 041 54 Košice, Slovakia

autor

Perhacova Zuzana

• Technical University in Zvolen, Faculty of Ecology and Environmental Sciences, Department of Biology and General Ecology, T. G. Masaryka 24, 960 53 Zvolen, Slovakia

autor

Vlcko Ladislav

• Technical University in Zvolen, Faculty of Ecology and Environmental Sciences, Department of Biology and General Ecology, T. G. Masaryka 24, 960 53 Zvolen, Slovakia

autor

Sedlakova-Kadukova Jana

• Pavol Jozef Šafárik University in Košice, Faculty of Science, Institute of Biology and Ecology, Šrobárova 2, 041 54 Košice, Slovakia

autor

Kvasnova Simona

• Matej Bel University, Faculty of Natural Science, Department of Biology and Ecology, Tajovskeho 40, 974 01 Banská Bystrica, Slovakia

autor

Pristas Peter

• Pavol Jozef Šafárik University in Košice, Faculty of Science, Institute of Biology and Ecology, Šrobárova 2, 041 54 Košice, Slovakia
• Institute of Animal Physiology, Slovak Academy of Sciences, Košice 041 01, Slovakia

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).