Optimization and validation of a method based on derivatization with methylating agent followed by HPLC-DAD for determining dithiocarbamates residues

• Autorzy: Zhou L. , Xu J. , Luan L. , Ma J. , Gong Y. , Qin D. , Pan C.
• Języki publikacji: EN

Abstrakty

EN

Dithiocarbamates fungicides (DTCs) are worldwidely used fungicides. Residue analytical methods on DTCs are usually based on headspace gas chromatography, which are not much stable and precise. In this study, a specific, simple and reliable method for determining DTCs fungicides residues was optimized and validated. The DTCs in foods and soils were extracted with an alkaline solution of EDTA and L-cysteine, followed by pH adjusting and methyl derivatization in methyl iodine solution. The organic layer of the reactants was separated, concentrated under vacuum and reconstructed in acetonitrile. DTCs residues were eluted on a C18 column and detected by HPLC-DAD at 272 nm. The S-alkyl derivatives of thiram, mancozeb and propineb were separated at different retention times. At fortified levels of 0.05 mg/kg to 2 mg/kg (residue expressed as CS2, in mg/kg, the same below), it is found that recoveries for DTCs spiked in apple, cucumber, tomato, rice and soil samples ranged from 70.8% to 105.3%, with relative standard deviations (RSD) from 0.6% to 13.7%. Limits of detection (LODs) and quantification (LOQs) ranged from 0.003 to 0.026 mg/kg and from 0.011 to 0.105 mg/kg for various foods and soils. This method was also applied to real sample tests.

Słowa kluczowe

EN

dithiocarbamates; methyl derivatization; pesticide residue; HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 4
• Strony: 613--625
• Opis fizyczny: Bibliogr. 17 poz., rys., tab.

Twórcy

autor

Zhou L.

• China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China

autor

Xu J.

• China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China

autor

Luan L.

• China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China

autor

Ma J.

• China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China

autor

Gong Y.

• Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China

autor

Qin D.

• Ministry of Agriculture Institute for the Control of Agrochemicals Beijing 100026 China

autor

Pan C.

• China Agricultural University Department of Applied Chemistry, College of Science Beijing 100193 China

Bibliografia

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