Implementation of ATP Biolumenescence Method in the Study of the Fungal Deterioration of Textile Artefacts

• Autorzy: Ljaljević Grbić M , Unković N , Stupar M , Vukojević J , Nedeljković T

Warianty tytułu

PL

Wdrażanie metody bioluminescencyjnej w badaniach deterioracji zabytków jedwabnych poprzez działanie grzybów

• Języki publikacji: EN

Abstrakty

EN

Two silk artefacts from museum collections in Serbia, a silk icon from Petar Lubarda Legacy and a scroll made with traditional japanese techniques were analyzed for fungal presence. Fungi isolated from both the silk artefacts belonged to the genera Aspergillus, Chaetomium, Penicillium and Rhizopus. Actively growing colonies of Aspergillus and Penicillium species had caused chromatic alterations on the silk icon and their metabolic activity was demonstrated by high relative light unit values. In this research, measurement of the total microbial activity using the ATP bioluminescence method was used for the first time on silk artefacts and on textile in general. Scaning electron microscopy analysis of the scroll indicated that hyphae of Chaetomium globosum are capable of the mechanical deterioration of silk, as shown by the presence of cracks and gaps in silk fibres. This appears to be the first report of silk fibre degradation caused by C. globosum.

PL

Badano dwa jedwabne zabytki z kolekcji muzealnej w Serbii. Wyizolowano grzyby należące do rodzajów Aspergillus, Chaetomium, Penicillium i Rhizopus. Rosnące aktywnie kolonie Aspergillus i Penicillium spowodowały zmiany chromatyczne. Z analizy SEM wynika, iż niezależnie od zmian barwnych stwierdzono również uszkodzenia mechaniczne w postaci szczelin i pęknięć. Po raz pierwszy udało się zastosować metodę bioluminescencyjną przy badaniu tekstyliów. Są to pierwsze doniesienia o oddziaływaniu grzybów tego typu na tkaniny jedwabne.

Słowa kluczowe

EN

ATP; biodeterioration; silk fibres; Chaetomium globosum; microfungi; SEM

PL

metoda bioluminescencyjna; badanie tekstyliów; SEM; działanie grzybów; Chaetomium globosum

• Wydawca: Sieć Badawcza Łukasiewicz - Łódzki Instytut Technologiczny
• Czasopismo: Fibres & Textiles in Eastern Europe
• Rocznik: 2014
• Tom: Vol. 22, no. 6 (108)
• Strony: 132--137
• Opis fizyczny: Bibliogr. 22 poz., rys., tab.

Twórcy

autor

Ljaljević Grbić M

• Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

autor

Unković N

• Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

autor

Stupar M

• Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

autor

Vukojević J

• Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

autor

Nedeljković T

• Central Institute for Conservation, Belgrade, Serbia

Bibliografia

• 1. Szostak-Kotowa J. Biodeterioration of textiles. International Biodeterioration & Biodegradation 2004; 53: 165-170.
• 2. Vollrath F. Biology of spider silk. International Journal of Biological Macromolecules 1999; 24: 81-88.
• 3. Carboni P. Silk: Biology, Chemistry and Technology. London, UK: Chapman & Hall, 1952.
• 4. Seves A, Romanò M, Maifreni T, et al. The microbial degradation of silk: a laboratory investigation. International Biodeterioration & Biodegradation 1998; 42: 203-211.
• 5. Otterburn MS. The chemistry and reactivity of silk. In: Asquith RS (ed.) The Chemistry of Natural Fibres. Ed. London: Plenum Press, New York, 1997, pp. 53-79.
• 6. Abdel-Kareem O. Monitoring, controlling and prevention of the fungal deterioration of textile artifacts in the museum of Jordanian Heritage. Mediterranean Archaeology and Archaeometry 2010; 10: 85-96.
• 7. Tiano P. Biodegradation of Cultural Heritage: Decay Mechanisms and Control Methods. In: ARIADNE Workshop 9 - Historic materials and their diagnostics. Prague, February 4-10, 2002.
• 8. Li M-Y, Zhao Y, Tong T, Hou X-H, Fang B-S, Wu S-Q, Shen X-Y, Tong H. Study of the degradation mechanism of Chinese historic silk (Bombyx mori) for the purpose of conservation. Polymer Degradation and Stability 2013; 98: 727-735.
• 9. Lundin A, Hasenson M, Persson J, Pousette A. Estimation of biomass in growing cell lines by Adenosine Triphosphate assay. Methods in Enzymology 1986; 133: 27-42.
• 10. BioControl. Lightning MVP system guide, 2005.
• 11. Samson RA, Hoekstra ES, Frisvad JC. Introduction to food- and airborne fungi. Ed. The Netherlands: Ponsen & Looyen, Wageningen, 2004.
• 12. Raper BK, Fennel DI. The Genus Aspergillus. Ed. Maryland: The Williams and Wilkins Company, Baltimore, 1965.
• 13. Pitt JI. The genus Penicillium and its teleomorphic state Eupenicillium and Talaromyces. Ed. UK: Academic Press, London, 1979.
• 14. Watanabe T. Soil and seed fungi. Morphologies of cultured fungi and key to species. Ed. London: Crc press, Boca Raton, 2002.
• 15. Urzì C, de Leo F. Sampling with adhesive tape strips: an easy and rapid method to monitor microbial colonization on monument surfaces. Journal of Microbiological Methods 2001; 44: 1-11.
• 16. Sirt E. Evaluation of biodeterioration in Nemrut mount monument and temple of Augustus by using various techniques. Master thesis, Middle East Techical University, Turkey, 2011.
• 17. Florian M-LE. Fungal facts: Solving fungal problems in heritage collections. Ed. UK: Archetype Publications LtD, London, 2002.
• 18. Heyes TF, Holden HS. The action of microorganisms on silk. Journal of the Society of Chemical Industry 1926; 45: T262-265.
• 19. Sato M. The effects of molds on fibres and their products. VIII. Scanning electron microscopic study on the destruction of silk yarns damaged by molds. Kyoto-furitsu Daigaku Gakujutsu Hokoku: Rigaku, Seikatsu Kagaku 1976, 27, pp. 59-64. Chemical Abstracts vol. 87, 202944t, 1977.
• 20. Popescu C-M, Tibirna CM, Manoliu A. Microscopic study of lime wood decayed by Chaetomium globosum. Cellulose Chemistry and Technology 2011; 45: 565-569.
• 21. Kuruppillai RV, Hersh SP, Tucker PA. Degradation of silk by heat and light. In: Williams JC (ed.) Historic Textile and Paper Materials; Conservation and Characterization. Advances in Chemistry Series no. 212. DC: American Chemical Society, Washington, 1989, pp. 111-127.
• 22. Ciferri O. The microbial degradation of silk: a laboratory investigation. In: Mitchel R, McNamara CJ (ed.) Cultural heritage microbiology: fundamental studies in conservation science. DC: ASM Press, Washington, 2010.