Radioisotopic investigation of crosslinking density in bovine pericardium used as a biomaterial

• Autorzy: Turek A. , Cwalina B. , Kobielarz M.
• Języki publikacji: EN

Abstrakty

EN

Stabilized bovine pericardium (BP) belongs to tissues routinely applied in production of heart valves. Commercial products are manufactured from tissues crosslinked by glutaraldehyde (GA). Dye-mediated photooxidation was also proposed as an alternative method, which allows for the elimination of clinical failures of GA-treated tissues. The aim of the study was to investigate the density of BP stabilized by GA and the methylene blue-mediated photooxidation, as compared with a native tissue. Crosslinking density was evaluated based on their ability to accumulate radioactive cobalt ions (60Co2+) and the permeability to these ions. Radioactivity was examined using a γ-spectrophotometer (Packard). The results showed the changes in the crosslinking density between the native tissues and photooxidized or GA-crosslinked tissues. Significant decreases in radioactivity were detected only in the thinnest tissues after photooxidation and in filtrates penetrating the same samples. Photooxidized pericardium of a larger thickness did not reveal any significant changes. Weight-dependence for the permeability was observed in the case of filtrates penetrating the GA-treated tissues. However, 60Co2+-accumulation in these samples remained at the same level. Photooxidation may lead to obtaining biomaterials with advantageous properties, i.e. a decreased calcium-binding capacity. Photooxidation efficiencies were dependent both on compactness and thickness of tissues and on process duration. It should be emphasized that the tissues’ structure after photooxidation was characterized by lower density. This may point to the presence of crosslinks of a smaller compactness in comparison with GA treatment. It has been shown that the factor indeed influencing the result of crosslinking is tissue thickness.

Słowa kluczowe

EN

bioprostheses; bovine pericardium (BP); crosslinking; methylene blue (MB); photooxidation; radioisotopic investigations

• Wydawca: Institute of Nuclear Chemistry and Technology
• Czasopismo: Nukleonika
• Rocznik: 2013
• Tom: Vol. 58, No. 4
• Strony: 511--517
• Opis fizyczny: Bibliogr. 44 poz., rys.

Twórcy

autor

Turek A.

• Department of Biopharmacy, Medical University of Silesia, 1 Narcyzów Str., 41-200 Sosnowiec, Poland, Tel.: [phone], Fax: [phone]
• Centre of Polymer and Carbon Materials, Polish Academy of Sciences, 34 M. Curie-Skłodowskiej Str., 41-819 Zabrze, Pola

autor

Cwalina B.

• Department of Environmental Biotechnology, Silesian University of Technology, 2 Akademicka Str., 44-100 Gliwice, Poland

autor

Kobielarz M.

• Division of Biomedical Engineering and Experimental Mechanics, Wrocław University of Technology, 7/9 Łukasiewicza Str., 50-371 Wrocław, Poland

Bibliografia

• 1. Au V, Madison SA (2000) Effects of singlet oxygen on the extracellular matrix protein collagen: oxidation of the collagen crosslink histidinohydroxylysinonorleucyne and histidine. Arch Biochem Biophys 384:133–142
• 2. Bianco RW, Phillips R, Mrachec J, Witson J (1996) Feasibility evaluation of a new pericardial bioprosthesis with dye mediated photo-oxidized bovine pericardial tissue. J Heart Valve Dis 5:317–322
• 3. Bodega F, Zocchi L, Agostoni E (2000) Macromolecule transfer through mesothelium and connective tissue. J Appl Physiol 89:2165–2173
• 4. Butterfield M, Fisher J (2000) Fatigue analysis of clinical bioprosthetic heart valves manufactured using photooxidized bovine pericardium. J Heart Valve Dis 9:161–166
• 5. Carpentier A, Lemaigre G, Robert L, Carpentier S, Dubost C (1969) Biological factors affecting long-term results of valvular heterografts. J Thorac Cardiov Sur 58:467–483
• 6. Cheung DT, Nimni ME (1982) Mechanism of crosslinking of proteins by glutaraldehyde. I. Reaction with model compounds. Connect Tissue Res 10:187–199
• 7. Cheung DT, Nimni ME (1982) Mechanism of crosslinking of proteins by glutaraldehyde. II. Reaction with monomeric and polymeric collagen. Connect Tissue Res 10:201–216
• 8. Cheung DT, Perelman N, Ko EC, Nimni ME (1985) Mechanism of crosslinking of proteins by glutaraldehyde. III. Reactions with collagen in tissues. Connect Tissue Res 13:109–115
• 9. Cwalina B, Bogacz A, Turek A (2000) The influence of proteins modification on pericardial tissue permeability to cobalt ions. In: Panuszka R, Iwaniec M, Reron E (eds) Wave methods and mechanics in biomedical engineering. Polish Acoustical Society, Kraków, pp 115–118
• 10. Cwalina B, Turek A, Miskowiec M, Nawrat Z, Domal- -Kwiatkowska D (2002) Biochemical stability of pericardial tissues modified using glutaraldehyde or formaldehyde. Engineering of Biomaterials 23/25:64–67
• 11. Cwalina B, Turek A, Nozynski J, Jastrzebska M, Nawrat Z (2005) Structural changes in pericardium tissue modified with tannic acid. Int J Artif Organs 28:648–653
• 12. DeLustro F, Sehl L, Estridge T, Wallace D (2004) Collagen: The next generation of resorbable biodevices in surgery. In: Yaszemski MJ, Trantolo DJ, Lewandrowski KU, Hasirci V, Altobelli DE, Wise DL (eds) Tissue engineering and novel delivery systems. Marcel Dekker, New York, pp 521–541
• 13. Dreesmann L, Hajosch R, Ahlers M, Nuernberger JV, Schlosshauer B (2008) Permeability testing of biomaterial membranes. Biomed Mater 3:034119
• 14. Fujimori E (1965) Ultraviolet light-induced change in collagen macromolecules. Biopolymers 3:115–119
• 15. Gelse K, Pöschl E, Aigner T (2003) Collagens – structure, functions, and biosynthesis. Adv Drug Deliver Rev 55:1531–1546
• 16. Goissis G, Yoshioka SA, Braile DM, Ramirez VD (1998) The chemical protecting group concept applied in crosslinking of natural tissues with glutaraldehyde acetals. Artif Organs 22:210–214
• 17. Golomb G, Schoen FJ, Smith MS, Linden J, Dixon M, Levy RJ (1987) The role of glutaraldehyde-induced crosslinks in calcification of bovine pericardium used in cardiac valve bioprostheses. Am J Pathol 127:122–130
• 18. Hetherington VJ, Kawalec JS, Dockery DS, Targoni OS, Lehmann PV, Nadler D (2005) Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors. BMC Musculoskelet Disord 205 Jun 29;6:36, doi: 10.1186/1471- -2474-6-36
• 19. Huang-Lee LL, Cheung DT, Nimni ME (1990) Biochemical changes and cytotoxicity associated with the degradation of polymeric glutaraldehyde derived crosslinks. J Biomed Mater Res 24:1185–1201
• 20. Jastrzebska M, Barwinski B, Mróz I, Turek A, Zalewska- -Rejdak J, Cwalina B (2005) Atomic force microscopy investigation of chemically stabilized pericardium tissue. Eur Phys J E Soft Matter 16:381–388
• 21. Jastrzebska M, Cwalina B, Zalewska-Rejdak J, Wilczok T, Kocot A (2001) Dielectric spectroscopy of chemically stabilized pericardium tissue at sub-zero temperatures. IEEE Trans Dielectr Electr Insul 8:527–530
• 22. Jastrzebska M, Zalewska-Rejdak J, Wrzalik R et al. (2006) Tannic acid-stabilized pericardium tissue: IR spectroscopy, atomic force microscopy, and dielectric spectroscopy investigations. J Biomed Mater Res A 78:148–156
• 23. Jayakrishnan A, Jameela SR (1996) Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices. Biomaterials 17:417–484
• 24. Jenkins CL, Raines RT (2002) Insights on the conformational stability of collagen. Nat Prod Rep 19:49–59
• 25. Jorge-Herrero E, Fonseca C, Barge AP et al. (2010) Biocompatibility and calcification of bovine pericardium employed for the construction of cardiac bioprostheses treated with different chemical crosslink methods. Artif Organs 34:E168–176
• 26. Lee JM, Langdon SE (1996) Thickness measurement of soft tissue biomaterials: a comparison of five methods. J Biomech 29:829–832
• 27. Levy RJ, Schoen FJ, Anderson HC et al. (1991) Cardiovascular implant calcification: a survey and update. Biomaterials 12:707–714
• 28. Meade KR, Silver FH (1990) Immunogenicity of collagenous implants. Biomaterials 11:176–180
• 29. Mechanic GL (1994) Cross-linking collagenous product. Patent US 5332475
• 30. Migneault I, Dartiguenave C, Bertrand MJ, Waldron KC (2004) Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking. Biotechniques 37:790–796
• 31. Moczar M, Mazzucotelli JP, Bertrand P, Ginat M, Leandri J, Loisance D (1994) Deterioration of bioprosthetic heart valves. ASAIO J 40:M697–701
• 32. Moore MA, Chen WM, Phillips RE, Bohachevsky IK, McIlroy BK (1996) Shrinkage temperature versus protein extraction as a measure of stabilization of photooxidized tissue. J Biomed Mater Res 32:209–214
• 33. Myers PO, Tissot C, Christenson JT, Cikirikcioglu M, Aggoun Y, Kalangos A (2010) Aortic valve repair by cusp extension for rheumatic aortic insufficiency in children: Long-term results and impact of extension material. J Thorac Cardiov Sur 140:836–844
• 34. Naimark WA, Lee JM, Limeback H, Cheung DT (1992) Correlation of structure and viscoelastic properties in the pericardial of four mammalian species. Am J Physiol 263;4(Pt 2):H1095–1106
• 35. Remi E, Khelil N, Di Centa E et al. (2011) Pericardial processing: challenges, outcomes and future prospects. In: Pignatello R (ed) Biomaterials science and engineering. InTech, Rijeka, pp 437–456
• 36. Rosenthal JT, Shaw BW Jr, Hardesty RL, Griffith BP, Starzl TE, Hakala TR (1983) Principles of multiple organ procurement from cadaver donors. Ann Surg 198:617–621
• 37. Simionescu D, Simionescu A, Deac R (1993) Mapping of glutaraldehyde-treated bovine pericardium and tissue selection for bioprosthetic heart valves. J Biomed Mater Res 27:697–704
• 38. Thoma RJ, Phillips RE (1995) The role of material surface chemistry in implant device calcification: a hypothesis. J Heart Valve Dis 4:214–221
• 39. Turek A, Cwalina B (2010) Some irradiation-influenced features of pericardial tissues engineered for biomaterials. In: Campolo D (ed) New development in biomedical engineering. InTech, Vukovar, pp 543–568
• 40. Turek A, Kobielarz M, Cwalina B, Bedzinski R (2010) Influence of methylene blue-mediated photooxidation on mechanical properties of porcine pericardium. Engineering of Biomaterials 96/98:99–103
• 41. Turek A, Marcinkowski A, Trzebicka B, Cwalina B, Dzierżewicz Z (2009) Nanostructure of bovine pericardium treated with trypsin. Engineering of Biomaterials 89/91:30–32
• 42. Weil L, Gordon WG, Burchert AR (1951) Photooxidation of amino acids in the presence of methylene blue. Arch Biochem Biophys 33:90–109
• 43. Westaby S, Bianco RW, Katsumata T, Termin P (1999) The carbomedics “Oxford” photofix stentless valve (PSV). Semin Thorac Cardiovasc Surg 11:206–209
• 44. Yoshioka SA, Goissis G (2008) Thermal and spectrophotometric studies of new crosslinking method for collagen matrix with glutaraldehyde acetals. J Mater Sci-Mater M 19:1215–1223