Endothelial cell proteome changed by contact with surfaces of biomaterials

• Autorzy: Komorowski P. , Jerczyńska H. , Pawłowska Z. , Walkowiak M. , Walkowiak B.
• Języki publikacji: EN

Abstrakty

EN

Biomaterials used for medical implants or instruments production can cause numerous undesirable effects in human organism. They may affect cells being in a direct contact with them and can cause changes in genes expression, and as a consequence, also in protein profile of these cells. The aim of the present work was to examine an effect of medical steel 316L, poly-paraxylylene (Parylene) and nanocrystalline diamond (NCD) surfaces on protein expression in human endothelial cell line EA.hy 926.Cells were grown in Dulbecco’s MEM (DMEM) supplemented with antibiotics (penicillin and streptomycin), glucose, 10% heat inactivated fetal bovine serum and HAT-supplement. After 48h of incubation cells were washed with PBS and treated with lysis buffer (7M urea, 2M thiourea, 4% CHAPS, 2 % IPG buffer pH 4-7, 1% DTT). Proteins were purified from cell lysates with 2-D CleanUp Kit, and concentration was assessed with 2D Quant Kit. After overnight rehydration of IEF strips (pH 4-7, 11cm), in the presence of purified proteins, isoelectric focusing procedure was performed until 40kVh. Then, stripes were equilibrated, and focused proteins were separated in 12,5% polyacrylamide gels (SDS PAGE). Silver stained gels were recorded with ImageScanner and analyzed with ImageMaster 2D Platinium 6.0 (GE Healthcare) software. Numerous changes in protein expression were detected in endothelial cells exposed to artificial surfaces of tested materials (see TABLE I).

Słowa kluczowe

EN

biomaterials; implants; cells

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2009
• Tom: Vol. 12, no. 89-91
• Strony: 30
• Opis fizyczny: tab.

Twórcy

autor

Komorowski P.

• Departament of Biophysics, Technical University of Lodz

autor

Jerczyńska H.

• Department of Molecular and Medical Biophysics, Medical University of Lodz, Poland

autor

Pawłowska Z.

• Department of Molecular and Medical Biophysics, Medical University of Lodz, Poland

autor

Walkowiak M.

• Departament of Biophysics, Technical University of Lodz

autor

Walkowiak B.

• Departament of Biophysics, Technical University of Lodz
• Department of Molecular and Medical Biophysics, Medical University of Lodz, Poland