Optimization of differentiation time of mesenchymal-stem-cell to tenocyte under a cyclic stretching with a microgrooved culture membrane and selected measurement cells

• Autorzy: Morita Y. , Yamashita T. , Toku Y. , Yu Y.

Identyfikatory

DOI

10.5277/ABB-00927-2017-04

• Języki publikacji: EN

Abstrakty

EN

There is a need for efficient stem cell-to-tenocyte differentiation techniques for tendon tissue engineering. More than 1 week is required for tenogenic differentiation with chemical stimuli, including co-culturing. Research has begun to examine the utility of mechanical stimuli, which reduces the differentiation time to several days. However, the precise length of time required to differentiate human bone marrow-derived mesenchymal stem cells (hBMSCs) into tenocytes has not been clarified. Understanding the precise time required is important for future tissue engineering projects. Therefore, in this study, a method was developed to more precisely determine the length of time required to differentiate hBMSCs into tenocytes with cyclic stretching stimulus. Methods: First, it had to be determined how stretching stimulation affected the cells. Microgrooved culture membranes were used to suppress cell orientation behavior. Then, only cells oriented parallel to the microgrooves were selected and evaluated for protein synthesis levels for differentiation. Results: The results revealed that growing cells on the microgrooved membrane and selecting optimally-oriented cells for measurement improved the accuracy of the differentiation evaluation, and that hBMSCs differentiated into tenocytes in approximately 10 h. Conclusions: The differentiation time corresponded to the time required for cellular cytoskeleton reorganization and cellular morphology alterations. This suggests that cells, when subjected to mechanical stimulus, secrete mRNAs and proteins for both cytoskeleton reorganization and differentiation.

Słowa kluczowe

EN

cyclic stretch; differentiation; differentiation time; human bone marrow-derived mesenchymal stem cell; hBMSC; mechanical stimulus; tenocyte

PL

różnicowanie; kości; stymulacja mechaniczna; hBMSC

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2018
• Tom: Vol. 20, no. 1
• Strony: 3--10
• Opis fizyczny: Bibliogr. 30 poz., rys., tab., wykr.

Twórcy

autor

Morita Y.

• Department of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University, Japan

autor

Yamashita T.

• Department of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University, Japan

autor

Toku Y.

• Department of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University, Japan

autor

Yu Y.

• Department of Micro-Nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University, Japan

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).