Excessive tensile strain induced the change in chondrocyte phenot

• Autorzy: Zhong D. , Chen X. , Luo Z.-P.

Identyfikatory

DOI

10.5277/ABB-01133-2018-02

• Języki publikacji: EN

Abstrakty

EN

Chondrocyte extracellular matrix type II collagen and proteoglycans ensure an important compression-bearing structure in synovial joint. However, much more type I collagen is generated in osteoarthritis, which implies the presence of abnormal tensile strain in cartilage. We hypothesize that tensile stress influences chondrocyte phenotype at the cellular level, leading to potential osteoarthritis. Methods: Chondrocytes were stimulated with cyclic excessive tensile (10%) or mild tensile or compressive strain (5%) at 0.5 Hz, 3 h per day for 3 days. Chondrocyte morphology and matrix proteoglycans level was separately determined by Rhodamine phalloidin and toluidine blue staining. The expression of cartilage marker molecules was measured using quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assays. Results: Chondrocytes demonstrated significant fibroblastic morphology, reduced proliferation and increased apoptosis following exposure to 10% tensile strain. The 10% tensile strain group induced the lowest matrix proteoglycans level. It observably reduced the expression of COL2A1, Acan and SOX9, and increased COL1A1 expression level. The 5% tensile (5% compression) group, maintained the chondrocyte phenotype. Conclusions: The findings identified the effects of different magnitudes of tensile stress on chondrocyte phenotype compared to compressive strain. Further studies on cartilage biomechanical micro-environment might benefit from this study.

Słowa kluczowe

EN

osteoarthritis; phenotype; chondrocytes; tensile strain

PL

osteoartroza; fenotyp; chondrocyt

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2018
• Tom: Vol. 20, no. 2
• Strony: 3--10
• Opis fizyczny: Bibliogr. 26 poz., rys., tab., wykr.

Twórcy

autor

Zhong D.

• Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, China

autor

Chen X.

• Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, China

autor

Luo Z.-P.

• Orthopedic Institute, Medical College, Soochow University, Suzhou, Jiangsu, China

Bibliografia

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Uwagi

This project is funded by the National Natural Science Foundation of China (81320108018, 31570943 and 31270995), Innovation and Entrepreneurship Program of Jiangsu Province, and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).