Effect of high voltage on the development of the plant tissue

• Autorzy: Hutová E.V. , Marcoň P. , Bartušek K.

Identyfikatory

DOI

10.5604/20830157.1176572

Warianty tytułu

PL

Wpływ wysokiego napięcia na rozwój tkanki roślinnej

• Języki publikacji: EN

Abstrakty

EN

In our experiment the electrical parameters that affect early somatic embryos (ESEs) were investigated. High voltage was generated by a special high voltage generator. High voltages ranging from 5 to 20 kV and frequency of 1 Hz were applied longitudinal and transversal directly on the Petri dish with 2 days old ESEs of Picea abies for periods of 3 hours every day. One Petri dish was placed directly on top of the high voltage generator and on the other Petri dish were fixed two copper plates for transmission of high voltage. Petri dishes were exposed to high voltage for 14 days. After this time, the influence of high voltage was evaluated. To evaluate the experiment were used biological and chemical methods, which confirmed the changes in the growth of ESEs.

PL

W naszym eksperymencie badane były parametry elektryczne, które wpływają na wczesne zarodki somatyczne (ESE). Wysokie napięcie wytworzono za pomocą specjalnego generatora. Wysokie napięcie, z przedziału od 5 do 20 kV i częstotliwości 1 Hz przykładano podłużnie i poprzecznie bezpośrednio do szalki Petriego z 2 dniowymi wczesnymi zarodkami somatycznymi Picea abies, codziennie na okres 3 godzin. Jedną szalkę umieszczano bezpośrednio na górnej części generatora wysokiego napięcia, a na drugiej szalce umieszczono dwie płytki miedziane do przenoszenia wysokiego napięcia. Szalki Petriego poddano działaniu wysokiego napięcia przez 14 dni. Po upływie tego czasu oceniano wpływ wysokiego napięcia. Do oceny zastosowano metody biologiczne i chemiczne. Potwierdziły one zmiany we wzroście wczesnych zarodków somatycznych (ESE).

Słowa kluczowe

EN

early somatic embryos; high voltage; Picea abies

PL

wczesne zarodki somatyczne; wysokie napięcia; Picea abies

• Wydawca: Wydawnictwo Politechniki Lubelskiej
• Czasopismo: Informatyka, Automatyka, Pomiary w Gospodarce i Ochronie Środowiska
• Rocznik: 2015
• Tom: nr 4
• Strony: 38--41
• Opis fizyczny: Bibliogr. 26 poz., rys., tab.

Twórcy

autor

Hutová E.V.

• Brno University of Technology, DTEE FEEC

autor

Marcoň P.

• Brno University of Technology, DTEE FEEC

autor

Bartušek K.

• Institute of Scientific Instruments, ASCR

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