TLC-DPPH• activity-guided separation and LC-DAD-MS identification of antioxidant compounds from Mutellina purpurea L. herb

• Autorzy: Sieniawska E. , Baj T. , Dudka J. , Mroczek T. , Głowniak K.
• Języki publikacji: EN

Abstrakty

EN

Many phytochemical investigations have been focused recently on the antioxidant activity of herbal extracts which can be used in phytotherapy. The previous study revealed antioxidative properties of Mutellina purpurea extract, but the constituents responsible for this action were not described yet. The aim of this study was activityguided separation and identification of antioxidant compounds from M. purpurea herb. Thin-layer chromatography-2,2-diphenyl-1-picrylhydrazyl (TLC-DPPH•) assay was used to detect compounds of interest; liquid chromatography-diode array detection-mass spectrometry (LC-DAD-MS) analysis allowed to identify antioxidants. The active fractions analyzed with LC-DAD-MS contained as a main compound chlorogenic acid accompanied with p-coumaric acid, ferulic acid, three dicaffeoylquinic acids, and caffeoylferuloylquinic acid. The fast TLC-DPPH• assay with LC-DAD-MS identification enabled the accurate identification of antioxidants in M. purpurea herb, which was done for the first time.

Słowa kluczowe

EN

antioxidant; Mutellina purpurea; polyphenolic compounds; DPPH; LC-DAD-MS analysis

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2016
• Tom: Vol. 28, no. 1
• Strony: 51--58
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Sieniawska E.

• Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki Str, PL-20-093 Lublin, Poland

autor

Baj T.

• Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki Str, PL-20-093 Lublin, Poland

autor

Dudka J.

• Medical Biology Unit, Medical University of Lublin, 8 Jaczewskiego Str, PL-20-090 Lublin, Poland

autor

Mroczek T.

• Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki Str, PL-20-093 Lublin, Poland

autor

Głowniak K.

• Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki Str, PL-20-093 Lublin, Poland

Bibliografia

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.