Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged and uncharged ferroelectric crystal surfaces

• Autorzy: Vandrovcova M. , Bacakova L. , Vanek P. , Petzelt J.
• Języki publikacji: EN

Abstrakty

EN

The cell-material interaction is significantly influenced by the physicochemical properties of the material surface, including its electrical charge. In this study, the effect of the surface polarity of ferroelectric LiNbO3 single crystals on the adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells was investigated. The cells were cultured on the normal-to-plane poled and in-plane poled plates resulting in positive, negative and zero surface charge. The number of initially adhering cells on day 1 after seeding, their spreading, shape, and their metabolic activity, production of type I collagen, activity of alkaline phosphatase and mineralization in the following days of cultivation (days 6 and 20) were comparable on all three tested surfaces. However, significant differences were found in the expression of mRNA for type I collagen, alkaline phosphatase and osteocalcin, i.e. an early, medium-term and late arkers of osteogenic cell differentiation, respectively. On day 20, the expression of type I collagen was significantly lower in cells on negatively-charged than on non-charged surfaces. Moreover, the expression of alkaline phosphatase and osteocalcin was higher in cells on positively-charged than on negatively-charged surfaces. These differences were generally more pronounced in standard cell culture medium than in osteogenic medium, which could, at least partly, mask the influence of the material surface properties on the cell behaviour. Thus, positively-charged LiNbO3 surfaces seemed to be more suitable for the osteogenic differentiation of bone marrow mesenchymal stem cells than the negatively-charged surfaces.

Słowa kluczowe

EN

electroactive ceramics; surface charge; cell number; resazurin; type I collagen; alkaline phosphatase; osteocalcin; bone matrix mineralization

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2016
• Tom: Vol. 19, no. 135
• Strony: 2--7
• Opis fizyczny: Bibliogr. 12 poz., tab., wykr., zdj.

Twórcy

autor

Vandrovcova M.

• Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Cz-14220 Prague, Czech Republic

autor

Bacakova L.

• Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, Cz-14220 Prague, Czech Republic

autor

Vanek P.

• Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, Cz-18221 Prague, Czech Republic

autor

Petzelt J.

• Institute of Physics of the Czech Academy of Sciences, Na Slovance 2, Cz-18221 Prague, Czech Republic

Bibliografia

• [1] Bacakova L., Filova E., Parizek M., Ruml T., Svorcik V.: Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants. Biotechnol Adv 29 (2011) 739-767.
• [2] Bacakova L., Filova E., Liskova J., Kopova I., Vandrovcova M., Havlikova J.: Nanostructured materials as substrates for the adhesion, growth, and osteogenic differentiation of bone cells. In: Nanobiomaterials in Hard Tissue Engineering. Applications of Nanobiomaterials. Volume 4, Ed. A. M. Grumezescu, Elsevier Inc., William Andrew Publishing, Oxford, Cambridge, 2016; pp. 103-153, ISBN 978-0-323-42862-0.
• [3] Schneider G.B., English A., Abraham M., Zaharias R., Stanford C., Keller J.: The effect of hydrogel charge density on cell attachment. Biomaterials 25 (2004) 3023-3028.
• [4] Kuwabara A., Hori N., Sawada T., Hoshi N., Watazu A., Kimoto K.: Enhanced biological responses of a hydroxyapatite/TiO2 hybrid structure when surface electric charge is controlled using radiofrequency sputtering. Dent Mater J 31 (2012) 368-376.
• [5] Tarafder S., Bodhak S., Bandyopadhyay A., Bose S.: Effect of electrical polarization and composition of biphasic calcium phosphates on early stage osteoblast interactions. J Biomed Mater Res B Appl Biomater 97 (2011) 306-314.
• [6] Nakamura S., Kobayashi T, Yamashita K. Numerical osteobonding evaluation of electrically polarized hydroxyapatite ceramics. J Biomed Mater Res A 68 (2004) 90-94.
• [7] Gao W, Feng B., Lu X., Wang J., Qu S., Weng J.: Characterization and cell behavior of titanium surfaces with PLL/DNA modification via a layer-by-layer technique. J Biomed Mater Res A 100 (2012) 2176-2185.
• [8] Wolf-Brandstetter C., Hempel U., Clyens S., Gandhi A.A., Korostynska O., Oswald S., Tofail S.A., Theilgaard N., Wiesmann H.P., Scharnweber D.: The impact of heat treatment on interactions of contact-poled biphasic calcium phosphates with proteins and cells. Acta Biomater 8 (2012) 3468-3477.
• [9] Jirka I., Vandrovcova M., Frank O., Tolde Z., Plšek J., Luxbacher T., Bacakova L., Stary V.: On the role of Nb-related sites of an oxidized β-TiNb alloy surface in its interaction with osteoblast-like MG-63 cells. Mater Sci Eng C Mater Biol Appl. 33(3) (2013) 1636-1645.
• [10] Vandrovcova M., Jirka I., Novotna K., Lisa V., Frank O., Kolska Z., Stary V., Bacakova L.: Interaction of human osteoblast-like Saos-2 and MG-63 cells with thermally oxidized surfaces of a titanium-niobium alloy. PLoS One 9 (2014) e100475.
• [11] Vanek P., Kolska Z., Luxbacher T., García J.A.L., Lehocky M., Vandrovcova M., Bacakova L., Petzelt J.: Electrical activity of ferroelectric biomaterials and its effects on the adhesion, growth and enzymatic activity of human osteoblast-like cells. J Phys D: Appl Phys 49 (2016) 175403.
• [12] Li J.J., Kawazoe N., Chen G.: Gold nanoparticles with different charge and moiety induce differential cell response on mesenchymal stem cell osteogenesis. Biomaterials 54 (2015) 226-236.

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.