Isolation of homoisoflavonoids from the fibrous roots of Ophiopogon japonicus by recycling high-speed counter-current chromatography and online antioxidant activity assay

• Autorzy: Zhou Yifeng , Wang Liling , Liu Tiebing , Mao Zedong , Ge Qing , Mao Jianwei

Identyfikatory

DOI

10.1556/1326.2018.00509

• Języki publikacji: EN

Abstrakty

EN

Two pairs of homoisoflavonoid analogues, 6-aldehydo-isoophiopogonanone A (1) / 6-aldehydo-isoophiopogonanone B (2) and methylophiopogonanone A (3) / methylophiopogonanone B (4), were obtained from the fibrous roots of Ophiopogon japonicus (L. f.) Ker-Gawl. (FROJ) by silica gel column chromatography (SGCC) and recycling high-speed counter-current chromatography (rHSCCC). First, the ethyl acetate fraction from the 70% ethanol extract was pre-separated by SGCC with a petroleum ether–ethyl acetate gradient (50:1–2:1, v/v). Then, the two sub-fractions containing homoisoflavonoid analogues were further separated by rHSCCC with n-hexane–ethyl acetate–methanol–acetonitrile–water (3:2:3.5:1:0.5, v/v) and n-hexane–ethyl acetate–methanol–acetonitrile–water (3:2:2.5:1:1.5, v/v). Finally, 6-aldehydo-isoophiopogonone A (16 mg), 6-aldehydo-isoophiopogonanone B (26 mg), methylophiopogonanone A (46 mg), and methylophiopogonanone B (148 mg) were obtained with purities of 97.82%, 96.70%, 97.76%, and 94.62%. Their structures were identified by high-resolution quadrupole-time-of-flight mass spectrometry (HR-QTOF-MS), ultraviolet (UV), and nuclear magnetic resonance spectroscopy (1H-NMR and 13C-NMR). These results demonstrated that rHSCCC could be used for the large scale preparation of homoisoflavonoid analogues from FROJ, which provides scientific support for utilization of untraditional medicinal part of O. japonicus and also for reduction in waste of plant resources. Additionally, an online antioxidant activity assay was investigated with hyphenated HSCCC-DPPH (1-diphenyl-2-picrylhydrazyl) radical scavenging detection.

Słowa kluczowe

EN

Ophiopogon japonicus; fibrous roots; homoisoflavonoid analogues; column chromatography; recycling high-speed counter-current chromatography; online antioxidant activity

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2019
• Tom: Vol. 31, no. 4
• Strony: 272--279
• Opis fizyczny: Bibliogr. 40 poz., rys., tab.

Twórcy

autor

Zhou Yifeng

• School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China
• Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Produces, Hangzhou, 310023, China
• Zhejiang Province Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacuring, 310023, China

autor

Wang Liling

• Zhejiang Academy of Forestry, Hangzhou, 310023, China.

autor

Liu Tiebing

• School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China
• Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Produces, Hangzhou, 310023, China
• Zhejiang Province Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacuring, 310023, China

autor

Mao Zedong

• School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China

autor

Ge Qing

• School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China
• Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Produces, Hangzhou, 310023, China
• Zhejiang Province Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacuring, 310023, China

autor

Mao Jianwei

• School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China
• Zhejiang Provincial Key Lab for Chemical and Biological Processing Technology of Farm Produces, Hangzhou, 310023, China
• Zhejiang Province Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacuring, 310023, China

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).