Potential applications of SOS-GFP biosensor to in vitro rapid screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in surface water

• Autorzy: Matejczyk M. , Rosochacki S. J.

Identyfikatory

DOI

10.12911/22998993/595

• Języki publikacji: EN

Abstrakty

EN

Escherichia coli K-12 GFP-based bacterial biosensors allowed the detection of cytotoxic and genotoxic effect of anticancer drug– cyclophosphamide and antidiabetic drug – metformin in PBS buffer and surface water. Experimental data indicated that recA::gfpmut2 genetic system was sensitive to drugs and drugs mixture applied in experiment. RecA promoter was a good bioindicator in cytotoxic and genotoxic effect screening of cyclophosphamide, metformin and the mixture of the both drugs in PBS buffer and surface water. The results indicated that E. coli K-12 recA::gfp mut2 strain could be potentially useful for first-step screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in water. Next steps in research will include more experimental analysis to validate recA::gfpmut2 genetic system in E. coli K-12 on different anticancer drugs.

Słowa kluczowe

EN

SOS-gfp biosensor; cytotoxicity; genotoxicity; cyclophosphamide; metformin

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2015
• Tom: Vol. 16, nr 1
• Strony: 116--121
• Opis fizyczny: Bibliogr. 13 poz., tab.

Twórcy

autor

Matejczyk M.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, Wiejska 45E, 15-351 Bialystok, Poland

autor

Rosochacki S. J.

• Department of Sanitary Biology and Biotechnology, Faculty of Civil Engineering and Environmental Engineering, Bialystok University of Technology, Wiejska 45E, 15-351 Bialystok, Poland

Bibliografia

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