Evaluation of changes caused by HR-HPV infection in squamous cell carcinoma of the head and neck using Raman microspectroscopy in combination with multivariate statistical analysis

Szurko, Agnieszka; Zygadło, Dorota; Student, Sebastian; Śnietura, Mirosław; Lorenc, Zbigniew; Kiełboń, Agnieszka; Waniczek, Dariusz

doi:10.2478/pjmpe-2024-0008

Artykuł - szczegóły

Tytuł artykułu

Evaluation of changes caused by HR-HPV infection in squamous cell carcinoma of the head and neck using Raman microspectroscopy in combination with multivariate statistical analysis

Autorzy

Szurko Agnieszka , Zygadło Dorota , Student Sebastian , Śnietura Mirosław , Lorenc Zbigniew , Kiełboń Agnieszka , Waniczek Dariusz

Wybrane pełne teksty z tego czasopisma

http://content.sciendo.com/view/journals/pjmpe/pjmpe-overview.xml

Identyfikatory

DOI

10.2478/pjmpe-2024-0008

Warianty tytułu

Języki publikacji

Abstrakty

Introduction: Squamous cell carcinoma of the head and neck region (HNSCC), with a positive status for high oncogenic potential human papillomavirus (HR-HPV), represents a clinically distinct disease entity compared to HPV-independent cases. Patients exhibit variations in prognosis and proposed therapy regimens. A prompt and reliable diagnosis of the presence of HPV infection could optimize the treatment for these patients. Currently employed treatment methods are long-term, expensive, and lack specificity, especially when administered separately. Material and methods: The research objective of this study is to explore significant differences in the Raman spectra of biological samples taken from patients with HNSCC, facilitating the confirmation of HPV virus presence. Study groups were delineated based on histopathological diagnosis and molecular biology tests, confirming the biological activity of the virus and the presence of the HR-HPV form with a diagnosis of a specific subtype. Results: To identify high oncogenic potential human papillomavirus (HR-HPV) infection as a crucial factor in squamous cell carcinoma of the head and neck region, an effective automatic data analysis system was established, relying on Raman microspectroscopy and multivariate analysis. Our results showed clear ranges of the Raman spectrum that differentiated between HPV-associated and non-HPV-associated cancers. Conclusions: In conclusion, our experience shows a great diagnostic potential of Raman confocal microscopy with multidimensional statistical analysis. In the future, the use of this method may allow for the creation of an effective and automated HR-HPV detection system in neoplastic tissue.

Słowa kluczowe

Raman spectroscopy human papilloma virus multivariate statistical analysis Squamous cell carcinoma of the head and neck region HNSCC

Wydawca

Polskie Towarzystwo Fizyki Medycznej
De Gruyter

Czasopismo

Polish Journal of Medical Physics and Engineering

Rocznik

2024

Tom

Vol. 30, Iss. 2

Strony

69--78

Opis fizyczny

Bibliogr. 51 poz., rys., tab.

Twórcy

autor

Szurko Agnieszka

agnieszka.szurko@us.edu.pl

Faculty of Science and Technology, University of Silesia, 75 Pułku Piechoty 1A, 41-500, Chorzów, Poland

https://orcid.org/0000-0002-1827-1955

autor

Zygadło Dorota

Faculty of Science and Technology, University of Silesia, 75 Pułku Piechoty 1A, 41-500, Chorzów, Poland

https://orcid.org/0000-0003-0462-0365

autor

Student Sebastian

Faculty of Automatic Control, Electronics and Computer Science, Silesian University of Technology, Gliwice, Poland
Biotechnology Center, Silesian University of Technology, Poland

https://orcid.org/0000-0003-1812-5496

autor

Śnietura Mirosław

Department of Pathomorphology and Molecular Diagnostics, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Katowice, Poland

https://orcid.org/0000-0002-3167-3326

autor

Lorenc Zbigniew

Department of General, Colorectal and Polytrauma Surgery, Faculty of Health Sciences in Katowice, Medical University of Silesia, Katowice, Poland

https://orcid.org/0000-0002-3192-4789

autor

Kiełboń Agnieszka

Faculty of Science and Technology, University of Silesia, 75 Pułku Piechoty 1A, 41-500, Chorzów, Poland

https://orcid.org/0009-0004-9112-1525

autor

Waniczek Dariusz

Department of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

https://orcid.org/0000-0002-0410-8604

Bibliografia

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Bibliografia

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