The technique of flow cytometry in diagnostic research

• Autorzy: Macheta A. , Radko K , Szymczyk S. , Podhorecka M
• Języki publikacji: EN

Abstrakty

EN

Flow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell scatters incident laser light and emits fluorescence. One of the most significant applications is immunophenotyping of cells - the most important tool in diagnosis and monitoring haematological disorders, such as acute and chronic leukemia, lymphoma, monoclonal gammopathy, myelodisplastic and myeloproliferative diseases.

Słowa kluczowe

EN

flow cytometry; immunophenotype; hematooncology

• Wydawca: Lublin University of Technology ; Polish Society of Ecological Engineering (PTIE), Branch of PTIE in Lublin
• Czasopismo: Advances in Science and Technology. Research Journal
• Rocznik: 2013
• Tom: Vol. 7, nr 19
• Strony: 55--59
• Opis fizyczny: Bibliogr. 19 poz., fig., tab.

Twórcy

autor

Macheta A.

• Department of Hematooncology and Bone Marrow Transplantation, Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, Poland

autor

Radko K

• Department of Hematooncology and Bone Marrow Transplantation, Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, Poland

autor

Szymczyk S.

• Students Scientific Association at Department of Hematooncology and Bone Marrow Transplantation Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, Poland

autor

Podhorecka M

• Department of Hematooncology and Bone Marrow Transplantation, Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, Poland

Bibliografia

• 1. Baj Z. Wykorzystanie cytometrii przepływowej w badanich płytek krwi. Central European J. of Immunology, 21, 1996, 119-127.
• 2. Baran J. Nowa epoka cytometrii przepływowej – przewodnik po współczesnych cytometrach i ich zastosowanie. Post. Biol. Kom., 24, 2008, 3-15.
• 3. Brown M, Wittwer C. Flow cytometry: principles and clinical applications in hematology. Clin. Chem., 46, 2000, 1221-1229.
• 4. Elghetany M.T., Patel J, Martinez J, Schwab H. CD87 as a Marker for Terminal Granulocytic Maturation: Assessment of its Expression During Granulopoiesis. Cytometry B, 51B, 2003, 9-13.
• 5. Fujimoto H, Sakata T, Hamaguchi Y, Shiga S, Tohyama K, Ichiyama S, Wang F, Houwen B. Flow Cytometric Method for Enumeration and Classification of Reactive Immature Granulocyte Populations. Cytometry, 42, 2000, 371-378.
• 6. Kaczmarek A, Osawa T, Leporowska E, Mackiewicz A. The role of flow cytometry in clinical diagnosis. Współcz. Onkol., 6, 2002, 366-373.
• 7. Kawiak J., Skierski J. S.: Cytometria przepływowa w badaniach komórek i niektóre jej zastosowania lekarskie. Post. Biol. Kom., 19, 1992, 2339-2354.
• 8. Mandy F.F., Bergron M, Minkus T. Principles of flow cytometry. Transfus. Sci. 16, 1995, 303-314.
• 9. Moldavan A. Photo-electric techniques for counting microscopical cells. Science, 88, 1934, 188-189.
• 10. Nunez R. Flow cytometry: principles and instrumentation. Curr. Issues Mol. Biol., 3, 2001, 39-45.
• 11. Owens M.A., Loken M.R. Flow cytometry principles for clinical laboratory practice. Quality assurance for quantitative immunophenotyping. Wiley- Liss, Inc. 1995.
• 12. Pituch-Noworolska A. Zastosowanie cytometrii przepływowej do diagnostyki białaczek i chłoniaków nieziarniczych. Central European J. of Immunology, 21, 1996, 138-146.
• 13. Rytwiński K, Wnuk A. Cytometria przepływowa, zasady działania, zastosowanie – ze szczególnym uwzględnieniem pediatrii. Medycyna Wieku Rozwojowego, 2, 1999, 281-290.
• 14. Sędek Ł, Mazur B. The application of monoclonal and policlonal antibodies in flow cytometry. Post. Biol. Kom., 24, 2008, 17-34.
• 15. Stopa P.J. The flow cytometry of Bacillus anthracis spores revisited. Cytometry, 41, 2000, 237-244.
• 16. Tucker F.T, O’Konsky C.T., Pickard H.B., Pitts J.N. A photoelectric counter for colloidal particles. J. Am. Chem. Soc., 69, 1947, 2422-2431.
• 17. Weiss D.J. The application of flow cytometric techniques to veterinary clinical hematology. Vet. Clin. Pathol., 31, 2002, 72-82.
• 18. Żeromski J, Sikora J, Dworacki G. Utility of flow cytometry in diagnostics and monitoring of lymphomas. Post. Biol. Kom., 24, 2008, 113-140.
• 19. Żydowicz G, Mazur B. Cells immunophenotype in normal hematopoiesis. Post. Biol. Kom., 24, 2008, 35-44.