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A densitometric HPTLC method for analysis of cordifolioside A both in 60% methanolic extract of Tinospora cordifolia and in a commercial formulation has been established and validated. Cordifolioside A was separated on aluminum-backed silica gel 60 F254 plates with chloroform-methanol 85:15 (%, v/v ) as mobile phase. A compact band was obtained for cordifolioside A at R F 0.52 ± 0.03. The limits of detection (LOD) and quantification (LOQ) were 20.12 and 60.36 ng per band, respectively. The highly precise and accurate method was used for analysis of cordifolioside A.
Słowa kluczowe
Czasopismo
Rocznik
Tom
Strony
683--692
Opis fizyczny
Bibliogr. 12 poz., rys., tab.
Twórcy
autor
- King Saud University Department of Pharmacognosy, College of Pharmacy, Al-Kharj King Saud Kingdom of Saudi Arabia
autor
- Jamia Hamdard, Hamdard Nagar Department of Pharmacognosy and Phytochemistry New Delhi 110062 India
autor
- Ranbaxy Research Laboratories Herbal Drug Research (Analytical Development) Gurgaon, Haryana India
autor
- Al-Arab Medical University Department of Pharmaceutics, Faculty of Pharmacy Benghazi Libya
Bibliografia
- [1] S.S. Singh, S.C. Pandey, S. Srivastava, V.S. Gupta, B. Patro, and A.C. Ghosh, Ind. J. Pharmacol., 35 , 83 (2003)
- [2] S. Kumar, N.S. Verma, D. Pande, and P. Srivastava, J. Med. Arom. Plant Sci., 22 , 61 (2000)
- [3] V.D. Gangan, P. Pradhan, A.T. Sipahimalani, and A. Banerji, Phytochemistry, 37 , 781 (1994)
- [4] J.B. Hanumann, R.K. Bhatt, and B.K. Sabata, Phytochemistry, 25 , 1677 (1986)
- [5] A.K. Pathak, P.K. Agarwal, D.C. Jain, R.P. Sharma, and O.W. Howarth, Ind. J. Chem. Sec. B, 34 , 674 (1995)
- [6] R. Maurya and S.S. Handa, Phytochemistry, 49 , 1343 (1998)
- [7] S.N. Dixit and R.L. Khosa, Ind. J. Appl. Chem., 34 , 46 (1971)
- [8] Kapil and S. Sharma, J. Ethnopharmacol., 58 , 89 (1997)
- [9] International Conference on Harmonisation (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use: Harmonised Triplicate Guideline on Validation of Analytical Procedures: Methodology, Recommended for Adoption at Step 4 of the ICH Process on November 1996 by the ICH Steering Committee, IFPMA, Switzerland
- [10] S. Baboota, S. Faiyaz, A. Ahuja, J. Ali, S. Shafiq, and S. Ahmed, Acta Chromatogr., 18 , 116 (2007)
- [11] J. Ali, N. Ali, Y. Sultana, S. Baboota, and S. Faiyaz, Acta Chromatogr., 18 , 168 (2007)
- [12] M.A. Naushad, M. Aqil, F.J. Ahmad, A. Ali, M.S. Faisal, M. Rizwan, and S. Faiyaz, J. Anal. Chem., 63 , 965, (2008)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-a2293007-130c-4dbd-b373-7bde34691511