Life in Extreme Habitats : Diversity of Endolithic Microorganisms from Cold Desert Ecosystems of Eastern Pamir

• Autorzy: Khomutovska N. , Jerzak M. , Kostrzewska-Szlakowska I. , Kwiatowski J. , Suska-Malawska M. , Syczewski M. , Jasser I.

Identyfikatory

DOI

10.3161/15052249PJE2017.65.4.001

• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to identify cyanobacteria diversity in rock communities from the cold desert ecosystem in Eastern Pamir Mountains (Tajikistan) and assess if the rock type and rock`s porosity can be indicators of microbial diversity in this extreme environment. Seven samples were collected in July 2015 from hillsides (ca 4000-4500 m a.s.l.) of the Eastern Pamir Mountains. Petrographic and scanning microscopy (SEM) allowed for the characterization of the rocks inhabited by endolithic communities as granite, gneiss and limestone with variable porosity. Based on next-generation sequencing (NGS) of amplicon of V3-V4 hypervariable region of 16S rRNA gene, we established that Actinobacteria, Proteobacteria and Cyanobacteria dominated the endolithic communities of microorganisms in the rocks studied, which distinguishes these communities from those described for other cold arid regions. Chroococcidiopsis and Leptolyngbya were dominant genera in the cyanobacterial communities according to culture-dependent analysis, as well as microscopic analyses of endoliths scraps from the rocks. Culture-independent metagenomic analyses revealed that Microcoleus, Acaryochloris, Chroococcidiopsis and Thermosynechococcus reads were the most abundant from all reads and dominated interchangeably in the samples. Endolithic communities of microorganisms in the rocks from the cold desert shrubland of Eastern Pamir Mts. appear to be diverse and different from communities described for other cold deserts.

Słowa kluczowe

EN

16S rRNA; Actinobacteria; cold desert; Cyanobacteria; endolithic microorganisms; NGS; next-generation sequencing; Proteobacteria; V3-V4 region

PL

16S rRNA; 16S rybosomalny RNA; geny 16S rRNA; Actinobacteria; promieniowce; zimna pustynia; Cyanobacteria; sinice; endolityczne mikroorganizmy; NGS; sekwencjonowanie nowej generacji; Proteobacteria; proteobakterie; region V3-V4

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2017
• Tom: Vol. 65, nr 4
• Strony: 303--319
• Opis fizyczny: Bibliogr. 27 poz., fot., rys., tab., wykr.

Twórcy

autor

Khomutovska N.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warszawa, Poland

autor

Jerzak M.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warszawa, Poland

autor

Kostrzewska-Szlakowska I.

• Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland

autor

Kwiatowski J.

• Department of Molecular Phylogenetics and Evolution, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warszawa, Poland

autor

Suska-Malawska M.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warszawa, Poland

autor

Syczewski M.

• Institute of Geochemistry, Mineralogy and Petrology, Faculty of Geology, University of Warsaw, Żwirki i Wigury 93, 02-089 Warszawa, Poland

autor

Jasser I.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089 Warszawa, Poland

Bibliografia

• [1] Bell R. A. 1993 — Cryptoendolithic algae of hot semiarid lands and deserts — J. Phycol. 29: 133-139, doi:10.1111/j.0022-3646.1993.00133.x.1993.
• [2] Billi D., Friedmann I. E., Hofer K. G., Grilli-Caiola M., Ocampo-Friedmann R. 2000 — Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis — Appl. Environ. Microbiol. 66: 1489-1492, doi: 10.1128/AEM.66.4.1489-1492.2000.
• [3] Crits-Christoph A., Robinson K. C., Ma B., Ravel J., Wierzchos J., Ascaso C., Artieda O., Souza-Egipsy V., Casero M. C., DiRuggiero J. 2016 — Phylogenetic and functional substrate specificity for endolithic microbial communities in hyper-arid environments — Appl. Frontiers Microbiol. 7: 301, doi: 10.3389/fmicb.2016.00301.2016.
• [4] De la Torre J. R., Goebel B. M., Friedmann E. I., Pace N. R. 2003 — Microbial diversity of cryptoendolithic communities from the McMurdo Dry Valleys, Antarctica — Appl. Environ. Microbiol. 69: 3858-3867, doi: 10.1128/AEM.69.7.3858-3867.2003.
• [5] De los Rios A., Wierzchos J., Sancho L. G., Ascaso C. 2004 — Exploring the physiological state of continental Antarctic endolithic microorganisms by microscopy FEMS — Appl. Microbiol. Ecol. 50: 143-152, doi:10.1016/j.femsec.2004.06.010.2004.
• [6] DiRuggiero J., Wierzchos J., Robinson C. K., Souterre T., Ravel J., Artieda O., Souza-Egipsy V., Ascaso C. 2013 — Microbial colonization of chasmoendolithic habitats in the hyper-arid zone of the Atacama Desert — Appl. Biogeosciences, 10: 2439-2450, doi:10.5194/bg-10-2439-2013.
• [7] Fenchel T. 2003 — Biogeography for bacteria — Appl. Science, 301: 925-926, doi: 10.1126/science.1089242.
• [8] Friedmann E. I. 1980 — Endolithic microbial life in hot and cold deserts — Appl. Origins Life Evol. Biosphere, 10: 223, doi: 10.1007/BF00928400.1980.
• [9] Friedmann E. I. 1982 — Endolithic microorganisms in the Antarctic cold desert — Science, 215: 1045-1053, doi: 10.1126/science.215.4536.1045.1982.
• [10] Friedmann E. I., Maosen H., Ocampo-Priedmann R. 1988 — Cryptoendolithic Lichen and Cyanobacterial Communities of the Ross Desert, Antarctica — Polarforschung, 58: 251-259.
• [11] Garcia-Pichel F., Prufert-Bebout L., Muyzer G. 1996 — Phenotypic and phylogenetic analyses show Microcoleus chtenoplastes to be a cosmopolitan cyanobacterium — Appl. Environ. Microbiol. 62: 3284-3291.
• [12] Golubic S., Friedmann I., Schneider J. 1981 — The Lithobiontic Ecological Niche, with Special Reference to Microorganisms — J. Sediment. Petrology, 51: 475-478.
• [13] Guillard, R. L., Lorenzen C. J. 1972 — Yellow-green algae with chlorophyllide — J. Phycol. 8: 10-14, doi: 10.1111/j.1529-8817.1972.tb03995.x.
• [14] Komárek J. 2013 — Cyanoprokaryota. 3. Heterocytous genera - (In: Süswasserflora von Mitteleuropa/Freshwater flora of Central Europe, Eds: B. Büdel, G. Gärtner, L. Krienitz, M. Schagerl) - Springer: Spektrum Berlin, Heidelberg, 113 pp.
• [15] Komárek J., Anagnostidis K. 1998 — Cyanoprokaryota 1. Chroococcales - (In: Süsswasserflora von Mitteleuropa 19/1, Eds: H. Ettl, G. Gärtner, H. Heynig, D. Mollenhauer) - Gustav Fischer, Jena-Stuttgart-Lübeck-Ulm, 548 pp.
• [16] Komárek J., Anagnostidis K. 2005 — Cyanoprokaryota. 2. Oscillatoriales - (In: Süsswasserflora von Mitteleuropa 19/2, Eds: B. Büdel, L. Krienitz, G. Gärtner, M. Schagerl) - Elsevier: Spektrum, Heidelberg, 759 pp.
• [17] Komárek J., Kaštovský J., Mareš J., Johansen J. R. 2014 — Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) using a polyphasic approach — Appl. Preslia, 86: 295-235.
• [18] Mętrak M., Sulwiński M., Chachulski Ł., Wilk M., Suska-Malawska M. 2015 — Creeping Environmental Problems in the Pamir Mountains: Landscape Conditions, Climate Change, wise Use and Threats (In: Climate Change Impacts on High-Altitude Ecosystems, Eds: M. Öztürk, K. Rehma) - Springer International Publishing, pp. 665-695.
• [19] Mętrak M., Chachulski Ł., Dovutsho D., Pawlikowski P., Rojan E., Sulwinski M., Suska-Malawska M. 2017 — Nature's patchwork: How water sources and soil salinity determine the distribution and structure of halophytic plant communities in arid environments of the Eastern Pamir — PlosOne, doi: 10.1371/journal.pone.0174496.
• [20] Pliński M., Komárek J. 2017 — Cyanobacteria of the Gulf of Gdańsk and the Southern Baltic — Wydawnictwo Uniwersytetu Gdańskiego, Gdańsk, 400 pp.
• [21] Pointing S. B., Chan Y., Lacap D. C., Lau M. C., Jurgens J. A., Farrell R. L. 2009 — Highly specialized microbial diversity in hyper-arid polar desert — Proc. Natl. Acad. Sci. U.S.A. 106: 19964-19969, doi: 10.1073/pnas.0908274106.
• [22] Rippka R., Deruelles J., Waterburg J. B, Herdman M., Stanier R. Y. 1979 — Generic assignments, strain histories and properties of pure culture of Cyanobacteria — Microbiology, 111: 1-61, doi: 10.1099/000221287-111-1-1.1979.
• [23] Sigler W., Bachofen R., Zeyer J. 2003 — Molecular characterization of endolithic cyanobacteria inhabiting exposed dolomite in central Switzerland — Appl. Environ. Microbiol. 5: 618-627, doi: 10.1046/j.1462-2920.2003.00453.2003.
• [24] Schwab, M., Ratschbacher, L., Siebel, W., McWilliams, M., Minaev, V., Lutkov, V., Chen, F., Stanek, K., Nelson, B., Frisch, W., and Wooden, J. L., 2004 — Assembly of the Pamirs: Age and origin of magmatic belts from the southern Tien Shan to the southern Pamirs and their relation to Tibet — Tectonics, v. 23, TC4002, doi: 10.1029/2003TC001583.
• [25] Wierzchos J., Ascaso C., McKay C. P. 2006 — Endolithic cyanobacteria in halite rocks from the hyperarid core of the Atacama Desert — Astrobiology, 6: 415-422, doi: 10.1089/ast.2006.6.415.2006.
• [26] Wong F. K., Lau M. C., Lacap D. C., Aitchison J. C., Cowan D. A., Pointing S. B. 2010 — Endolithic microbial colonization of limestone in a high-altitude arid environment — Appl. Microb. Ecol. 59: 689-699, doi: 10.1007/s00248-009-9607-8.2010.
• [27] Yung C. C. M., Chan Y., Lacap D. C., Pérez-Ortega S., de los Rios-Murillo A., Charles K., Lee S., Craig Cary S., Pointing B. 2014 — Characterization of Chasmoendolithic Community in Miers Valley, McMurdo Dry Valleys, Antarctica — Appl. Microb Ecol. 68: 351, doi: 10.1007/s00248-014-0412-7.2014.

Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).